BrightLine Basic single-band filter set, optimized for Blue Fluorescent Protein and other like fluorophores

Semrock Part Number:BFP-A-Basic-000

IDEX Part Number: S-000668  /

Semrock

This filter set is ideal for use with Blue Fluorescent Protein (BFP) and other UV-excited, blue-emission fluorophores, including DAPI. This hard-coated filter set is the best value on the market, delivering an optimal combination of high brightness and contrast.

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  • Price Not Available.
  • 935.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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We now offer LFOV (large field of view) cube sets compatible with Nikon Eclipse Ti2 microscopes. Please contact us for more information.
Set Components

FF01-390/18-25

FL-004665

Each
  • Tavg > 90% 380.8 – 399.3 nm
  • Center Wavelength of 390 nm and GMBW of 18.5 nm
  • FWHM of 22.4 nm

FL-004665

FF01-460/60-25

FL-004673

Each
  • Tavg > 90% 430.2 – 489.9 nm
  • Center Wavelength of 460 nm and GMBW of 59.7 nm
  • FWHM of 64.2 nm

FL-004673

FF416-Di01-25x36

FL-007089

Each
  • Dichroic Beamsplitter with edge at 416 nm
  • Ravg > 90% 360 – 407 nm
  • Tavg > 90% 425 – 575 nm
  •  Flatness / RWE: Standard Epi-fluorescence

Custom sizing delivery confirmed ARO (sizing fee applies).

FL-007089

Optical Specifications Value
General Filter Specifications Value
Optical Damage Rating Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.
Microscope Compatibility BrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Physical Filter Specifications   Value
Scratch-Dig 80-50

BFP-A-Basic-NQF

S-001160 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series

BFP-A-Basic-NTE

S-000035 / 0

Out of Stock Backordered In Stock

This set is delivered already mounted in microscope filter holder, often called 'cube', ready to be dropped into your microscope.
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator

BFP-A-Basic-OMF

S-001161 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81

BFP-A-Basic-ZHE

S-001103 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1

BFP-A-Basic-OFF

S-000036 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-FF for microscopes: BX53, BX63

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TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
1,8-ANS 372 477
Alexa Fluor® 350 343 441
Alexa Fluor® 405 401 422
AMCA (Aminomethylcoumarin) 350 448
ATTO 390 392 477
BD Horizon V450 406 449
BD Horizon V500 410 500
BFP (EBFP) 380 440
Cascade Blueâ„¢ 401 419
CellTrace calcein violet 400 452
DAPI 359 461
DEAC 432 472
DY-415 418 470
DyLight 405 398 420
ECFP 435 475
ER-Trackerâ„¢ Blue-White DPX 372 557
HiLyte Fluorâ„¢ 405 403 428
Hoechst 33258 352 455
Hoechst 33342 352 455
Hoechst 34580 392 440
LIVE-DEAD Fixable Violet Dead Cell Stain 403 455
LysoSensor Blue 374 424
Marina Blue 363 461
mBBr+GSH 394 490
Pacific Blueâ„¢ 404 455
sgBFPâ„¢ 387 451
Sirius 355 424
SpectrumBlue 405 449
TagBFP 402 457
Vybrant DyeCycle Violet 369 437

Specifications

BFP-A-Basic-NQF

S-001160 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series

BFP-A-Basic-NTE

S-000035 / 0

Out of Stock Backordered In Stock

This set is delivered already mounted in microscope filter holder, often called 'cube', ready to be dropped into your microscope.
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator