BrightLine^™ 2.0 single-band filter set, optimized for BFP and other like fluorophores

Semrock Part Number: BFP-A-000

IDEX Part Number: S-443949 /

FF01-392/21-25 FL-442703 Each T_avg > 95% 381 – 402 nm Center Wavelength of 391.5 nm and GMBW of 21 nm FWHM of 24.9 nm	FL-442703	1 Each
FF01-459/62-25 FL-442736 Each T_avg > 95% 428 – 490 nm Center Wavelength of 459 nm and GMBW of 62 nm FWHM of 66.6 nm	FL-442736	1 Each
FF415-Di01-25x36 FL-442765 Each Dichroic Beamsplitter with edge at 415 nm R_avg > 98% 350 – 402 nm T_avg > 95% 428 – 1100 nm Flatness / RWE: Standard Epi-fluorescence	FL-442765	1 Each

Optical Specifications	Value
Blocking	BrightLine® 2.0 filters have blocking far exceeding OD 8 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.
General Filter Specifications	Value
Optical Damage Rating	Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.
Physical Filter Specifications	Value
Scratch-Dig	80-50
Dichroic Orientation	Reflective coating side should face toward light source.

Topic	Description
Cleaning Optical Filters	Instructions for cleaning hard coated optical filters
Filter Reliability	Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density	Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO^™ option help?	Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.

Optimized – Filter set is specifically optimized for that fluorophore.

Compatible – Should result in nearly ideal performance in most situations.

Acceptable – Actual performance is dependent on specific experimental conditions.

Fluorophore	Peak Excitation Wavelength(nm)	Peak Emission Wavelength(nm)
1,8-ANS	372	477
Alexa Fluor^® 350	343	441
Alexa Fluor^® 405	401	422
AMCA (Aminomethylcoumarin)	350	448
ATTO 390	392	477
BD Horizon V450	406	449
BD Horizon V500	410	500
BFP (EBFP)	380	440
Cascade Blue™	401	419
CellTrace calcein violet	400	452
DAPI	359	461
DEAC	432	472
DY-415	418	470
DyLight 405	398	420
ECFP	435	475
ER-Tracker™ Blue-White DPX	372	557
HiLyte Fluor™ 405	403	428
Hoechst 33258	352	455
Hoechst 33342	352	455
Hoechst 34580	392	440
LIVE-DEAD Fixable Violet Dead Cell Stain	403	455
LysoSensor Blue	374	424
Marina Blue	363	461
mBBr+GSH	394	490
Pacific Blue™	404	455
sgBFP™	387	451
Sirius	355	424
SpectrumBlue	405	449
TagBFP	402	457
Vybrant DyeCycle Violet	369	437

Optical Specifications: Value
Blocking: BrightLine® 2.0 filters have blocking far exceeding OD 8 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.
General Filter Specifications: Value
Optical Damage Rating: Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.
Physical Filter Specifications: Value
Scratch-Dig: 80-50
Dichroic Orientation: Reflective coating side should face toward light source.

Topic	Description
Cleaning Optical Filters	Instructions for cleaning hard coated optical filters
Filter Reliability	Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density	Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO^™ option help?	Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.

FF01-392/21-25