This filter set is designed for use with Yellow Fluorescent Protein (YFP) and Venus and works well with other key fluorophores. The Avant™ filter set family delivers significant increases in fluorescence performance for small Stokes Shift fluorophores compared to standard filter sets. Steep spectral edges and high complementary OD blocking maximize signal while maintaining low bleedthrough and high signal to noise ratio. The new Precise Edge Placement capability ensures every filter set delivers consistent performance.
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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1,418.04 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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AF01-539/27-25
FL-426382 / 0
• Tavg > 93% 525.5 – 552.5 nm
• Center Wavelength of 539 nm and GMBW of 27 nm
• FWHM of 31 nm
• Center Wavelength of 539 nm and GMBW of 27 nm
• FWHM of 31 nm
AF01-504/24-25
FL-429408 / 0
• Tavg > 93% 492.5 – 516.5 nm
• Center Wavelength of 504 nm and GMBW of 24 nm
• FWHM of 28 nm
• Center Wavelength of 504 nm and GMBW of 24 nm
• FWHM of 28 nm
AF521-Di01-25x36
FL-429409 / 0
- Dichroic Beamsplitter with edge at 521 nm
- Ravg > 98% 470 – 516.5 nm
- Tavg > 93% 525.5 – 580 nm
- Flatness / RWE: Standard Epi-fluorescence
Custom sizing delivery confirmed ARO (sizing fee applies).
1 of 1
This set is mounted in a ZEISS Microscopy FL CUBE EC P&C Fluorescence Filter Holder, which is compatible with Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner, and Axio Scope A1, from ZEISS Microscopy.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 5.0 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| ATTO 488 | 500 | 525 |
|
|
| ATTO 520 | 516 | 538 |
|
|
| Venus | 516 | 528 |
|
|
| YFP (yellow GFP) | 513 | 530 |
|
total selected
Clear selection
Select all 3 products from all pages.
AF01-539/27-25
FL-426382 / 0
• Tavg > 93% 525.5 – 552.5 nm
• Center Wavelength of 539 nm and GMBW of 27 nm
• FWHM of 31 nm
• Center Wavelength of 539 nm and GMBW of 27 nm
• FWHM of 31 nm
AF01-504/24-25
FL-429408 / 0
• Tavg > 93% 492.5 – 516.5 nm
• Center Wavelength of 504 nm and GMBW of 24 nm
• FWHM of 28 nm
• Center Wavelength of 504 nm and GMBW of 24 nm
• FWHM of 28 nm
AF521-Di01-25x36
FL-429409 / 0
- Dichroic Beamsplitter with edge at 521 nm
- Ravg > 98% 470 – 516.5 nm
- Tavg > 93% 525.5 – 580 nm
- Flatness / RWE: Standard Epi-fluorescence
Custom sizing delivery confirmed ARO (sizing fee applies).
1 of 1
This set is mounted in a ZEISS Microscopy FL CUBE EC P&C Fluorescence Filter Holder, which is compatible with Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner, and Axio Scope A1, from ZEISS Microscopy.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 5.0 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
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FF562-Di02-25x36
FL-011618