Avant single-band filter set, optimized for Yellow Fluorescent Protein (YFP), Venus and other like fluorophores

Semrock Part Number: Avant-YFP-ZHE

IDEX Part Number: S-431321  /

This filter set is designed for use with Yellow Fluorescent Protein (YFP) and Venus and works well with other key fluorophores. The Avant™ filter set family delivers significant increases in fluorescence performance for small Stokes Shift fluorophores compared to standard filter sets. Steep spectral edges and high complementary OD blocking maximize signal while maintaining low bleedthrough and high signal to noise ratio. The new Precise Edge Placement capability ensures every filter set delivers consistent performance.
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  • 1,418.04 USD Each
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AF01-539/27-25

FL-426382 / 0

Out of Stock Backordered In Stock
• Tavg > 93% 525.5 – 552.5 nm
• Center Wavelength of 539 nm and GMBW of 27 nm
• FWHM of 31 nm

AF01-504/24-25

FL-429408 / 0

Out of Stock Backordered In Stock
• Tavg > 93% 492.5 – 516.5 nm
• Center Wavelength of 504 nm and GMBW of 24 nm
• FWHM of 28 nm

AF521-Di01-25x36

FL-429409 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 521 nm
  • Ravg > 98% 470 – 516.5 nm
  • Tavg > 93% 525.5 – 580 nm
  •  Flatness / RWE: Standard Epi-fluorescence

Custom sizing delivery confirmed ARO (sizing fee applies).

1 of 1

This set is mounted in a ZEISS Microscopy FL CUBE EC P&C Fluorescence Filter Holder, which is compatible with Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner, and Axio Scope A1, from ZEISS Microscopy.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm5.0 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.1 mm
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
ATTO 488 500 525
ATTO 520 516 538
Venus 516 528
YFP (yellow GFP) 513 530

Related Products

AF01-539/27-25

FL-426382 / 0

Out of Stock Backordered In Stock
• Tavg > 93% 525.5 – 552.5 nm
• Center Wavelength of 539 nm and GMBW of 27 nm
• FWHM of 31 nm

AF01-504/24-25

FL-429408 / 0

Out of Stock Backordered In Stock
• Tavg > 93% 492.5 – 516.5 nm
• Center Wavelength of 504 nm and GMBW of 24 nm
• FWHM of 28 nm

AF521-Di01-25x36

FL-429409 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 521 nm
  • Ravg > 98% 470 – 516.5 nm
  • Tavg > 93% 525.5 – 580 nm
  •  Flatness / RWE: Standard Epi-fluorescence

Custom sizing delivery confirmed ARO (sizing fee applies).

1 of 1

This set is mounted in a ZEISS Microscopy FL CUBE EC P&C Fluorescence Filter Holder, which is compatible with Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner, and Axio Scope A1, from ZEISS Microscopy.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm5.0 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.1 mm
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.