This filter set is designed for use with Cy3™ and Alexa Fluor 555 and works well with other orange fluorophores. The Avant™ filter set family delivers significant increases in fluorescence performance for small Stokes Shift fluorophores compared to standard filter sets. Steep spectral edges and high complementary OD blocking maximize signal while maintaining low bleedthrough and high signal to noise ratio. The new Precise Edge Placement capability ensures every filter set delivers consistent performance.
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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1,536.71 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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AF01-535/40-25
FL-426386 / 0
• Tavg > 93% 515 – 555 nm
• Center Wavelength of 535 nm and GMBW of 40 nm
• FWHM of 43.5 nm
• Center Wavelength of 535 nm and GMBW of 40 nm
• FWHM of 43.5 nm
AF01-584/40-25
FL-426477 / 0
• Tavg > 93% 564 – 604 nm
• Center Wavelength of 584 nm and GMBW of 40 nm
• FWHM of 44 nm
• Center Wavelength of 584 nm and GMBW of 40 nm
• FWHM of 44 nm
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AF560-Di01-25x36
FL-429439 / 0
- Dichroic Beamsplitter with edge at 560 nm
- Ravg > 98% 490 – 555 nm
- Tavg > 93% 564 – 680 nm
- Flatness / RWE: Standard Epi-fluorescence
Custom sizing delivery confirmed ARO (sizing fee applies).
1 of 1
This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept a 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| Alexa Fluor® 555 | 553 | 565 |
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| ATTO 550 | 553 | 576 |
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| Cy3™ | 554 | 566 |
|
total selected
Clear selection
Select all 3 products from all pages.
AF01-535/40-25
FL-426386 / 0
• Tavg > 93% 515 – 555 nm
• Center Wavelength of 535 nm and GMBW of 40 nm
• FWHM of 43.5 nm
• Center Wavelength of 535 nm and GMBW of 40 nm
• FWHM of 43.5 nm
AF01-584/40-25
FL-426477 / 0
• Tavg > 93% 564 – 604 nm
• Center Wavelength of 584 nm and GMBW of 40 nm
• FWHM of 44 nm
• Center Wavelength of 584 nm and GMBW of 40 nm
• FWHM of 44 nm
AF560-Di01-25x36
FL-429439 / 0
- Dichroic Beamsplitter with edge at 560 nm
- Ravg > 98% 490 – 555 nm
- Tavg > 93% 564 – 680 nm
- Flatness / RWE: Standard Epi-fluorescence
Custom sizing delivery confirmed ARO (sizing fee applies).
1 of 1
This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept a 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
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LP02-405RU-25
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