Avant^™ single-band filter set, optimized for Alexa Fluor 546, ATTO 550 and other like fluorophores

Semrock Part Number: Avant-AF546-000

IDEX Part Number: S-434167 /

AF01-551/22-25 FL-433417 Each • Tavg > 93% 540 – 562 nm • Center Wavelength of 551 nm and GMBW of 22 nm • FWHM of 27.4 nm	FL-433417	1 Each
AF01-592/42-25 FL-433416 Each • Tavg > 93% 571 – 613 nm • Center Wavelength of 592 nm and GMBW of 42 nm • FWHM of 45.9 nm	FL-433416	1 Each
AF567-Di01-25x36 FL-433418 Each Dichroic Beamsplitter with edge at 567 nm R_avg > 98% 510 – 562 nm T_avg > 93% 571 – 650 nm Flatness / RWE: Standard Epi-fluorescence Custom sizing delivery confirmed ARO (sizing fee applies).	FL-433418	1 Each

Optical Specifications	Value
Blocking	Avant filters have blocking far exceeding OD 5 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.
General Filter Specifications	Value
Optical Damage Rating	Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hrs.
Microscope Compatibility	Avant filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Physical Filter Specifications	Value
Orientation	Reflective surface marked with part number - Orient in direction of incoming light
Exciter/Emitter Orientation	Arrow on ring indicates preferred direction of propagation of light.

Topic	Description
Cleaning Optical Filters	Instructions for cleaning hard coated optical filters
Filter Reliability	Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density	Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO^™ option help?	Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.

Optimized – Filter set is specifically optimized for that fluorophore.

Compatible – Should result in nearly ideal performance in most situations.

Acceptable – Actual performance is dependent on specific experimental conditions.

Fluorophore	Peak Excitation Wavelength(nm)	Peak Emission Wavelength(nm)	Compatibility	Display on Plot above
Alexa Fluor^® 546	556	572
ATTO 550	553	576

Optical Specifications: Value
Blocking: Avant filters have blocking far exceeding OD 5 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.
General Filter Specifications: Value
Optical Damage Rating: Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hrs.
Microscope Compatibility: Avant filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Physical Filter Specifications: Value
Orientation: Reflective surface marked with part number - Orient in direction of incoming light
Exciter/Emitter Orientation: Arrow on ring indicates preferred direction of propagation of light.

Topic	Description
Cleaning Optical Filters	Instructions for cleaning hard coated optical filters
Filter Reliability	Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density	Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO^™ option help?	Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.

AF01-551/22-25