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Avant™ single-band filter set, optimized for Alexa Fluor 546, ATTO 550 and other like fluorophores

Semrock Part Number: Avant-AF546-000

IDEX Part Number: S-434167  /

Semrock


This filter set is designed for use with Alexa Fluor 546, ATTO 550 and works well with other like fluorophores. The Avant™ filter set family delivers significant increases in fluorescence performance for small Stokes Shift fluorophores compared to standard filter sets. Steep spectral edges and high complementary OD blocking maximize signal while maintaining low bleedthrough and high signal to noise ratio. The new Precise Edge Placement capability ensures every filter set delivers consistent performance.
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We now offer LFOV (large field of view) cube sets compatible with Nikon Eclipse Ti2 microscopes. Please contact us for more information.
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Set Components

AF01-551/22-25

FL-433417

Each
• Tavg > 93% 540 – 562 nm
• Center Wavelength of 551 nm and GMBW of 22 nm
• FWHM of 27.4 nm

FL-433417

AF01-592/42-25

FL-433416

Each
• Tavg > 93% 571 – 613 nm
• Center Wavelength of 592 nm and GMBW of 42 nm
• FWHM of 45.9 nm

FL-433416

AF567-Di01-25x36

FL-433418

Each
  • Dichroic Beamsplitter with edge at 567 nm
  • Ravg > 98% 510 – 562 nm
  • Tavg > 93% 571 – 650 nm
  •  Flatness / RWE: Standard Epi-fluorescence

Custom sizing delivery confirmed ARO (sizing fee applies).

FL-433418

Optical Specifications Value
Blocking Avant filters have blocking far exceeding OD 5 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.
General Filter Specifications Value
Optical Damage Rating Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hrs.
Microscope Compatibility Avant filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Physical Filter Specifications   Value
Orientation Reflective surface marked with part number - Orient in direction of incoming light
Exciter/Emitter Orientation Arrow on ring indicates preferred direction of propagation of light.
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO™ option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
LinkDescription
Request a DrawingLooking for an optical filter drawing file for this product? Request one now!
Request Custom Optical FiltersContact us today to start a conversation about custom optical filters.
Access Optical Filter WarrantyFind our warranty in our Terms & Conditions of Sale.
Semrock Optical Filters RoHS CertificateRoHS, stands for Restriction of Hazardous Substances. The current RoHS Directive currently restricts the use of ten substances.
Semrock Optical Filters REACH CertificateREACH is a regulation of the European Union, adopted to improve the protection of human health and the environment from the risks that can be posed by chemicals, while enhancing the competitiveness of the EU chemicals industry.
Download Optical Filters 101 GuideFind an in-depth overview of optical filter principles, specifications, and applications. Request a digital copy of this guide now.
Request Clean Filter Checklist PosterLooking to clean your optical filters? Need instructions? Request a printed copy of our Clean Filter Checklist Poster.
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
Alexa Fluor® 546 556 572
ATTO 550 553 576

Specifications

TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO™ option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
LinkDescription
Request a DrawingLooking for an optical filter drawing file for this product? Request one now!
Request Custom Optical FiltersContact us today to start a conversation about custom optical filters.
Access Optical Filter WarrantyFind our warranty in our Terms & Conditions of Sale.
Semrock Optical Filters RoHS CertificateRoHS, stands for Restriction of Hazardous Substances. The current RoHS Directive currently restricts the use of ten substances.
Semrock Optical Filters REACH CertificateREACH is a regulation of the European Union, adopted to improve the protection of human health and the environment from the risks that can be posed by chemicals, while enhancing the competitiveness of the EU chemicals industry.
Download Optical Filters 101 GuideFind an in-depth overview of optical filter principles, specifications, and applications. Request a digital copy of this guide now.
Request Clean Filter Checklist PosterLooking to clean your optical filters? Need instructions? Request a printed copy of our Clean Filter Checklist Poster.

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