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683 nm Avant™ Dichroic Beamsplitter

Semrock Part Number: AF683-Di01-25x36

IDEX Part Number: FL-426308  /

Semrock

For optimal filter set performance, pair this dichroic with AF01-653/47 and AF01-712/48, which have been optimized for Cy5.5™, Alexa Fluor 680, ATTO 665 and other key fluorophores. Precise edge placement of the steep transition edge combined with very high reflection and transmission bands maximize fluorophore performance compared to standard dichroics.
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  • 319.73 USD Each
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Optical Specifications Value
Transmission Band 1 Tavg > 93% 688 – 770 nm
Reflection Band 1 Ravg > 98% 600 – 677 nm
Edge Wavelength 1 683 nm
Flatness Standard Epi-Fluorescence
General Filter Specifications Value
Angle of Incidence 45 ± 1.5 degrees
Cone Half-angle 2 degrees
Optical Damage Rating Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hrs.
Flatness / RWE Classification   Standard Epi-fluorescence
Physical Filter Specifications   Value
Transverse Dimensions (L x W) 25.2 mm x 35.6 mm
Transverse Tolerance ± 0.1 mm
Clear Aperture ≥ 80% (elliptical)
Scratch-Dig 60-40
Filter Thickness (unmounted) 1.05 mm
Filter Thickness Tolerance (unmounted) ± 0.05 mm
Orientation Reflective surface marked with part number - Orient in direction of incoming light
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Laser Damage ThresholdProvides information for calculating laser damage threshold for pulsed and continuous wave lasers
BrightLine Multiphoton Fluorescence FiltersDescribes how multiphoton microscopy works and how optical filters for multiphoton fluorescence differ from traditional fluorescence filters.
Journal Article
TopicDescription
Optical Filters for Multiphoton MicroscopyCraig Hodgson, PhD, and Turan Erdogan, PhD, Semrock, Inc., BioPhotonics, November 2006
Describes how multiphoton microscopy works, several current applications and how optical filters for multiphoton fluorescence differ from traditional fluorescence filters
White Paper
TopicDescription
Flatness of Dichroic Beamsplitters Affects Focus and Image QualityAnalysis of how the radius of curvature of a dichroic filter affects focal plane shift, spot size and image fidelity in fluorescence microscopy.
Application
TopicDescription
Multiphoton MicroscopyAn overview of multiphoton microscopy, including solutions from Semrock and additional learning resources.

Specifications

TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Laser Damage ThresholdProvides information for calculating laser damage threshold for pulsed and continuous wave lasers
BrightLine Multiphoton Fluorescence FiltersDescribes how multiphoton microscopy works and how optical filters for multiphoton fluorescence differ from traditional fluorescence filters.
Journal Article
TopicDescription
Optical Filters for Multiphoton MicroscopyCraig Hodgson, PhD, and Turan Erdogan, PhD, Semrock, Inc., BioPhotonics, November 2006
Describes how multiphoton microscopy works, several current applications and how optical filters for multiphoton fluorescence differ from traditional fluorescence filters
White Paper
TopicDescription
Flatness of Dichroic Beamsplitters Affects Focus and Image QualityAnalysis of how the radius of curvature of a dichroic filter affects focal plane shift, spot size and image fidelity in fluorescence microscopy.
Application
TopicDescription
Multiphoton MicroscopyAn overview of multiphoton microscopy, including solutions from Semrock and additional learning resources.

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