For optimal filter set performance, pair this dichroic with AF01-504/24 and AF01-539/27, which have been optimized for Yellow Fluorescent Protein (YFP), Venus, ATTO 488, ATTO 514, ATO 520 and other key fluorophores. Precise edge placement of the steep transition edge combined with very high reflection and transmission bands maximize fluorophore performance compared to standard dichroics. |
Topic | Description |
Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
Flatness of Dichroic Beamsplitters | Describes the impact of non-flat dichroic beamsplitters on microscope image fidelity |
Laser Damage Threshold | Provides information for calculating laser damage threshold for pulsed and continuous wave lasers |
Topic | Description |
Flatness of Dichroic Beamsplitters Affects Focus and Image Quality | Analysis of how the radius of curvature of a dichroic filter affects focal plane shift, spot size and image fidelity in fluorescence microscopy. |
FL-011618