Semrock’s LED-based light engine filter sets are designed to use common excitation filters between single-band and corresponding multiband configurations to simplify interchangeability when installing the excitation filters into the light engine. This not only provides seamless transition between using single-band and multiband filter sets but also provides significant cost savings.
This filter set is optimized to simultaneously deliver bright signal and high signal-to-noise ratio (contrast) for measuring YFP (or other key fluorophores – see complete list above). This set is spectrally aligned for excitation with many of the broadband or discrete LED-based light engines available in the market.
Interested in learning how this filter set will perform with your light source? Try Semrock's SearchLight plotting & calculation tool.
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This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.
Filter Role | Filter Size | Maximum Thickness |
Exciter (Diameter) | 25.0 mm | 5.0 mm |
Emitter (Diameter) | 25.0 mm | 3.5 mm |
Dichroic (L × W) | 25.2 mm × 35.6 mm The Nikon “true” cube needs a Di with corner chamfered (-QF) | 1.1 mm (Can accept a 2 mm dichroic) |
Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
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5-carboxy-2,7-dichlorofluorescein | 500 | 525 |
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5-FAM (5-carboxyfluorescein) | 492 | 518 |
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6-carboxyrhodamine 6G | 525 | 555 |
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6-JOE | 520 | 548 |
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Acridine Orange (+DNA) | 502 | 526 |
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Alexa Fluor® 488 | 499 | 520 |
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Alexa Fluor® 500 | 503 | 525 |
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Alexa Fluor® 514 | 518 | 543 |
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Alexa Fluor® 532 | 534 | 553 |
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ATTO 488 | 500 | 525 |
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ATTO 495 | 495 | 527 |
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ATTO 520 | 516 | 538 |
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ATTO 532 | 501 | 545 |
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BODIPY R6G | 528 | 547 |
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Calcein | 494 | 514 |
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Calcium Green-1 | 507 | 529 |
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CyQUANT GR-DNA | 502 | 523 |
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DAF-FM-NO | 495 | 519 |
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Dronpa | 502 | 516 |
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DY-505-Phalloidin | 503 | 530 |
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DyLight 488 | 493 | 518 |
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Eosin | 525 | 546 |
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FITC (Fluorescein) | 495 | 519 |
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FlAsH-CCPFCC | 511 | 530 |
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Fluo-3 | 506 | 527 |
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Fluo-4 | 494 | 516 |
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Fluorescein dextran | 501 | 524 |
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Fluorescein-pH 8.0 | 489 | 517 |
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Fluoro-Emerald | 494 | 518 |
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FluoSpheres Orange fluorescent microspheres | 540 | 559 |
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FluoSpheres Yellow-Green fluorescent microspheres | 503 | 514 |
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FM 1-43 | 473 | 579 |
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Gold 525 | 521 | 551 |
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Green 496 | 496 | 520 |
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Green 500 | 501 | 524 |
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HCS LipidTOX Green phospholipidosis | 504 | 536 |
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HiLyte Fluorâ„¢ 488 | 497 | 526 |
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JO-JO-1, JO-PRO-1 | 530 | 544 |
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LIVE-DEAD Fixable Green Dead Cell Stain | 498 | 525 |
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LysoTracker Yellow HCK-123 | 488 | 565 |
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Magnesium Green | 507 | 531 |
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mHoneyDew | 478 | 562 |
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mOrange2 | 551 | 565 |
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NeuroTrace 500/525 Green Fluorescent Nissl Stain | 495 | 524 |
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Oregon Greenâ„¢ 488 | 498 | 526 |
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Oregon Greenâ„¢ 514 | 513 | 532 |
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PicoGreen® | 502 | 522 |
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Qdot® 525 Nanocrystals | 300 | 525 |
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Qdot® 545 Nanocrystals | 300 | 543 |
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Rhodamine 110 | 497 | 519 |
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Rhodamine 123 | 507 | 529 |
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Rhodamine Green | 497 | 523 |
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Rhodol Green | 497 | 524 |
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Sodium Green | 507 | 532 |
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SpectrumGreen | 497 | 538 |
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SYBR Gold nucleic acid gel stain-DNA | 496 | 539 |
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SYBR Green I nucleic acid gel stain-DNA | 498 | 522 |
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SYBR Safe DNA gel stain-DNA | 509 | 526 |
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SYTO 11 | 506 | 525 |
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SYTO RNASelect geen fluorescent cell stain | 503 | 527 |
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SYTOX Green-DNA | 504 | 524 |
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TO-PRO-1 | 515 | 531 |
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TOTO-1 | 514 | 531 |
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TurboYFP | 525 | 538 |
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Venus | 516 | 528 |
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VIC | 530 | 550 |
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Vybrant DyeCycle Green | 506 | 534 |
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Vybrant DyeCycle Orange | 519 | 563 |
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YFP (yellow GFP) | 513 | 530 |
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ZsYellow1 | 529 | 539 |
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This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.
Filter Role | Filter Size | Maximum Thickness |
Exciter (Diameter) | 25.0 mm | 5.0 mm |
Emitter (Diameter) | 25.0 mm | 3.5 mm |
Dichroic (L × W) | 25.2 mm × 35.6 mm The Nikon “true” cube needs a Di with corner chamfered (-QF) | 1.1 mm (Can accept a 2 mm dichroic) |