Semrock’s LED-based light engine filter sets are designed to use common excitation filters between single-band and corresponding multiband configurations to simplify interchangeability when installing the excitation filters into the light engine. This not only provides seamless transition between using single-band and multiband filter sets but also provides significant cost savings.
This filter set is optimized to simultaneously deliver bright signal and high signal-to-noise ratio (contrast) for measuring YFP (or other key fluorophores – see complete list above). This set is spectrally aligned for excitation with many of the broadband or discrete LED-based light engines available in the market.
Interested in learning how this filter set will perform with your light source? Try Semrock's SearchLight plotting & calculation tool.
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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1,554.39 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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FF01-509/22-25
FL-004258 / 0
- Tavg > 93% 497.6 – 519.5 nm
- Center Wavelength of 508.6 nm and GMBW of 21.9 nm
- FWHM of 26.1 nm
FF01-544/24-25
FL-004684 / 0
- Tavg > 93% 532 – 556 nm
- Center Wavelength of 544 nm and GMBW of 24 nm
- FWHM of 29.1 nm
FF526-Di01-25x36
FL-007012 / 0
- Dichroic Beamsplitter with edge at 526 nm
- Ravg > 98% 350 – 519.5 nm
- Tavg > 93% 532 – 950 nm
- Flatness / RWE: Standard Epi-fluorescence
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This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept a 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 5-carboxy-2,7-dichlorofluorescein | 500 | 525 |
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| 5-FAM (5-carboxyfluorescein) | 492 | 518 |
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| 6-carboxyrhodamine 6G | 525 | 555 |
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| 6-JOE | 520 | 548 |
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| Acridine Orange (+DNA) | 502 | 526 |
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| Alexa Fluor® 488 | 499 | 520 |
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| Alexa Fluor® 500 | 503 | 525 |
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| Alexa Fluor® 514 | 518 | 543 |
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| Alexa Fluor® 532 | 534 | 553 |
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| ATTO 488 | 500 | 525 |
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| ATTO 495 | 495 | 527 |
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| ATTO 520 | 516 | 538 |
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| ATTO 532 | 501 | 545 |
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| BODIPY R6G | 528 | 547 |
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| Calcein | 494 | 514 |
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| Calcium Green-1 | 507 | 529 |
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| CyQUANT GR-DNA | 502 | 523 |
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| DAF-FM-NO | 495 | 519 |
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| Dronpa | 502 | 516 |
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| DY-505-Phalloidin | 503 | 530 |
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| DyLight 488 | 493 | 518 |
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| Eosin | 525 | 546 |
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| FITC (Fluorescein) | 495 | 519 |
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| FlAsH-CCPFCC | 511 | 530 |
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| Fluo-3 | 506 | 527 |
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| Fluo-4 | 494 | 516 |
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| Fluorescein dextran | 501 | 524 |
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| Fluorescein-pH 8.0 | 489 | 517 |
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| Fluoro-Emerald | 494 | 518 |
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| FluoSpheres Orange fluorescent microspheres | 540 | 559 |
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| FluoSpheres Yellow-Green fluorescent microspheres | 503 | 514 |
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| FM 1-43 | 473 | 579 |
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| Gold 525 | 521 | 551 |
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| Green 496 | 496 | 520 |
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| Green 500 | 501 | 524 |
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| HCS LipidTOX Green phospholipidosis | 504 | 536 |
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| HiLyte Fluor™ 488 | 497 | 526 |
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| JO-JO-1, JO-PRO-1 | 530 | 544 |
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| LIVE-DEAD Fixable Green Dead Cell Stain | 498 | 525 |
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| LysoTracker Yellow HCK-123 | 488 | 565 |
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| Magnesium Green | 507 | 531 |
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| mHoneyDew | 478 | 562 |
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| mOrange2 | 551 | 565 |
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| NeuroTrace 500/525 Green Fluorescent Nissl Stain | 495 | 524 |
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| Oregon Green™ 488 | 498 | 526 |
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| Oregon Green™ 514 | 513 | 532 |
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| PicoGreen® | 502 | 522 |
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| Qdot® 525 Nanocrystals | 300 | 525 |
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| Qdot® 545 Nanocrystals | 300 | 543 |
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| Rhodamine 110 | 497 | 519 |
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| Rhodamine 123 | 507 | 529 |
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| Rhodamine Green | 497 | 523 |
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| Rhodol Green | 497 | 524 |
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| Sodium Green | 507 | 532 |
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| SpectrumGreen | 497 | 538 |
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| SYBR Gold nucleic acid gel stain-DNA | 496 | 539 |
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| SYBR Green I nucleic acid gel stain-DNA | 498 | 522 |
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| SYBR Safe DNA gel stain-DNA | 509 | 526 |
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| SYTO 11 | 506 | 525 |
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| SYTO RNASelect geen fluorescent cell stain | 503 | 527 |
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| SYTOX Green-DNA | 504 | 524 |
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| TO-PRO-1 | 515 | 531 |
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| TOTO-1 | 514 | 531 |
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| TurboYFP | 525 | 538 |
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| Venus | 516 | 528 |
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| VIC | 530 | 550 |
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| Vybrant DyeCycle Green | 506 | 534 |
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| Vybrant DyeCycle Orange | 519 | 563 |
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| YFP (yellow GFP) | 513 | 530 |
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| ZsYellow1 | 529 | 539 |
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total selected
Clear selection
Select all 3 products from all pages.
FF01-509/22-25
FL-004258 / 0
- Tavg > 93% 497.6 – 519.5 nm
- Center Wavelength of 508.6 nm and GMBW of 21.9 nm
- FWHM of 26.1 nm
FF01-544/24-25
FL-004684 / 0
- Tavg > 93% 532 – 556 nm
- Center Wavelength of 544 nm and GMBW of 24 nm
- FWHM of 29.1 nm
FF526-Di01-25x36
FL-007012 / 0
- Dichroic Beamsplitter with edge at 526 nm
- Ravg > 98% 350 – 519.5 nm
- Tavg > 93% 532 – 950 nm
- Flatness / RWE: Standard Epi-fluorescence
1 of 1
This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept a 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
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FF01-441/511/593/684/817-25
FL-410484