Semrock’s LED-based light engine filter sets are designed to use common excitation filters between single-band and corresponding multiband configurations to simplify interchangeability when installing the excitation filters into the light engine. This not only provides seamless transition between using single-band and multiband filter sets but also provides significant cost savings.
This 7-filter "Sedat" quad-band set is designed for high-speed, sequential imaging of DAPI, FITC, and Texas Red (and other key fluorophores – see complete list above). This set has been designed to deliver significant fluorescence signal improvements for LED-based light engines, including 365 nm DAPI LEDs, compared with traditional sets designed for standard broadband light sources such as mercury or xenon arc lamps. The complete set is comprised of a triple-band beamsplitter with three single-band emitters intended to be mounted in filter wheels and three single-band exciters which can be installed into the LED light engine or mounted in filter wheels.
Interested in learning how this filter set will perform with your light source? Try Semrock's SearchLight plotting & calculation tool.
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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2,810.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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Filter set mounted in microscope holders/cubes found below in related products section.
Set Components
FF01-378/52-25FL-004583
Each
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FL-004583 |
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FF01-432/36-25FL-004373
Each
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FL-004373 |
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FF01-474/27-25FL-004596
Each
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FL-004596 |
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FF02-525/40-25FL-004652
Each
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FL-004652 |
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FF02-641/75-25FL-004436
Each
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FL-004436 |
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FF03-575/25-25FL-004522
Each
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FL-004522 |
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FF409/493/596-Di02-25x36FL-007086
Each
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FL-007086 |
total selected
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LED-DA/FI/TX-3X3M-B-NQF
S-002892 / 0
This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series
LED-DA/FI/TX-3X3M-B-NTE
S-002893 / 0
This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator
LED-DA/FI/TX-3X3M-B-OMF
S-002896 / 0
This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81
LED-DA/FI/TX-3X3M-B-ZHE
S-405899 / 0
This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1
LED-DA/FI/TX-3X3M-B-OFF
S-002894 / 0
This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-FF for microscopes: BX53, BX63
LED-DA/FI/TX-3X3M-B-LDMK
S-002889 / 0
This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Leica DM-K for microscopes: DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, DM-6000, DMI6000 B, DM4 and DM6
LED-DA/FI/TX-3X3M-B-LDMP
S-002890 / 0
This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Leica DMi8 (P-Cube) for microscopes: DMi8
1 of 1
Blocking
BrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.
Microscope Compatibility
BrightLine filters are available to fit Leica, Nikon, Olympus and Zeiss microscopes.
Optical Damage Rating
Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.
Scratch-Dig
60-40
Exciter/Emitter Orientation
Arrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation
Reflective coating side should face toward light source and sample.
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
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| 2-dodecylresorufin-lipid | 582 | 595 |
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| 5-carboxynapthofluorescein (pH 10) | 555 | 615 |
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| 5-FAM (5-carboxyfluorescein) | 492 | 518 |
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| 5-ROX (carboxy-X-rhodamine) | 578 | 604 |
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| 7-AAD (7-aminoactinomycin D) | 548 | 648 |
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| Acridine Orange (+DNA) | 502 | 526 |
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| Acridine Yellow | 470 | 550 |
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| Alexa Fluor® 405 | 401 | 422 |
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| Alexa Fluor® 430 | 431 | 541 |
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| Alexa Fluor® 488 | 499 | 520 |
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| Alexa Fluor® 500 | 503 | 525 |
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