BrightLine® single-band filter set, optimized for DAPI and other like fluorophores, illuminated with 365 to 395 nm LED-based light engines

Semrock Part Number: LED-DAPI-B-OFX

IDEX Part Number: S-001693  /

Semrock

Semrock’s LED-based light engine filter sets are designed to use common excitation filters between single-band and corresponding multiband configurations to simplify interchangeability when installing the excitation filters into the light engine. This not only provides seamless transition between using single-band and multiband filter sets but also provides significant cost savings.

This filter set is optimized to simultaneously deliver bright signal and high signal-to-noise ratio (contrast) for measuring DAPI (or other key fluorophores – see complete list above). This set is spectrally aligned for excitation with many of the broadband or discrete LED-based light engines available in the market. Interested in learning how this filter set will perform with your light source? Try Semrock's SearchLight plotting & calculation tool.

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  • Each
    US Domestic pricing – contact your local distributor for other currency list prices
  • Price Not Available.
  • 1,764.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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Filter Role Filter Size Maximum Thickness
Exciter (Diameter)32.0 mm5.0 mm
Emitter (Diameter)32.0 mm3.5 mm
Dichroic (L × W)32.0 mm × 44.0 mm
(Corners must me cut for OFX cube)
1.5 mm
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
1,8-ANS 372 477
Alexa Fluor® 405 401 422
Aqua 431 430 478
ATTO 390 392 477
BD Horizon V450 406 449
BFP (EBFP) 380 440
Cascade Blue™ 401 419
CellTrace calcein violet 400 452
CFP (ECFP) 433 475
DAPI 359 461
DY-415 418 470
DyLight 405 398 420
ECFP 435 475
HiLyte Fluor™ 405 403 428
LIVE-DEAD Fixable Violet Dead Cell Stain 403 455
LysoSensor Blue 374 424
Marina Blue 363 461
mBBr+GSH 394 490
Pacific Blue™ 404 455
sgBFP™ 387 451
SpectrumBlue 405 449
TagBFP 402 457
Vybrant DyeCycle Violet 369 437

Standard Component Sizes

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)32.0 mm5.0 mm
Emitter (Diameter)32.0 mm3.5 mm
Dichroic (L × W)32.0 mm × 44.0 mm
(Corners must me cut for OFX cube)
1.5 mm