BrightLine® full-multiband filter set, optimized for CFP, YFP, & mCherry and other like fluorophores, illuminated with LED-based light engines

Semrock Part Number: LED-CFP/YFP/mCherry-A-NTE-ZERO

IDEX Part Number: S-000312  /

Semrock

This Full-multiband triple-band set is designed for imaging CFP, YFP, and mCherry (and other key fluorophores – see complete list above). This state-of-the-art set represents a break-through in triple-band performance, and has been optimized for popular LED-based light engines. This set has been designed to deliver significant fluorescence signal improvements for LED-based light engines compared with traditional sets designed for standard broadband light sources such as mercury or xenon arc lamps. The complete set is comprised of a triple-band beamsplitter, emitter, and exciter filter, all with "no burn-out" performance.

Interested in learning how this filter set will perform with your light source? Try Semrock's SearchLight plotting & calculation tool.

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    US Domestic pricing – contact your local distributor for other currency list prices
  • Price Not Available.
  • 2,108.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm3.5 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.1 mm
(Can accept 2 mm dichroic)
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
2-dodecylresorufin-lipid 582 595
5-carboxy-2,7-dichlorofluorescein 500 525
5-carboxynapthofluorescein (pH 10) 555 615
5-FAM (5-carboxyfluorescein) 492 518
5-ROX (carboxy-X-rhodamine) 578 604
6-carboxyrhodamine 6G 525 555
6-JOE 520 548
7-AAD (7-aminoactinomycin D) 548 648
Acridine Orange (+DNA) 502 526
Acridine Yellow 470 550
Alexa Fluor® 488 499 520
Alexa Fluor® 500 503 525
Alexa Fluor® 514 518 543
Alexa Fluor® 532 534 553
Alexa Fluor® 568 579 603
Alexa Fluor® 594 590 618
Alexa Fluor® 660 663 691
APC (Allophycocyanin) 651 660
Aqua 431 430 478
AsRed 2 576 592
ATTO 425 436 484
ATTO 465 453 507
ATTO 488 500 525
ATTO 495 495 527
ATTO 520 516 538
ATTO 532 501 545
ATTO 565 563 592
ATTO 590 594 624
ATTO 594 601 624
ATTO 610 614 634
BD Horizon V500 410 500
BOBO™-1 461 484
BOBO™-3 570 605
BODIPY R6G 528 547
BODIPY TR-X phallacidin 590 621
Calcein 494 514
Calcium Crimson 589 609
Calcium Green-1 507 529
Carboxynaphthofluorescein 599 674
CellTrace BODIPY TR methyl ester 598 625
CellTracker Red CMTPX 586 614
Cerulean 434 473
CFP (ECFP) 433 475
Cy3.5™ 578 591
CyQUANT GR-DNA 502 523
DAF-FM-NO 495 519
DDAO 648 657
Dronpa 502 516
DY-415 418 470
DY-505-Phalloidin 503 530
DY-590 582 599
DY-630 634 656
DyLight 488 493 518
DyLight 594 593 618
ECFP 435 475
Eosin 525 546
FITC (Fluorescein) 495 519
FlAsH-CCPFCC 511 530
Fluo-3 506 527
Fluo-4 494 516
Fluorescein dextran 501 524
Fluorescein-pH 8.0 489 517
Fluoro-Emerald 494 518
FluoSpheres Crimson fluorescent microspheres 620 646
FluoSpheres Orange fluorescent microspheres 540 559
FluoSpheres Red fluorescent microspheres 577 607
FluoSpheres Yellow-Green fluorescent microspheres 503 514
FM 1-43 473 579
FM 4-64 505 725
Gold 525 521 551
Green 496 496 520
Green 500 501 524
HcRed1 588 618
HCS LipidTOX Deep Red neutral lipid stain 635 652
HCS LipidTOX Green phospholipidosis 504 536
HCS LipidTOX Red neutral lipid stain 582 616
HCS LipidTOX Red phospholipidosis 584 608
HiLyte Fluor™ 488 497 526
HiLyte Fluor™ 594 592 616
JO-JO-1, JO-PRO-1 530 544
KFP-Red 575 599
LDS 751 560 711
LIVE-DEAD Fixable Green Dead Cell Stain 498 525
LIVE-DEAD Fixable Red Dead Cell Stain 595 613
LysoTracker Red 573 592
LysoTracker Yellow HCK-123 488 565
Magnesium Green 507 531
mApple 566 594
mBBr+GSH 394 490
mCherry 587 610
mHoneyDew 478 562
MitoTracker™ Red 578 598
mPlum 589 649
mRFP1 584 700
mRuby 558 605
mStrawberry 574 596
mTFP1 462 492
NeuroTrace 500/525 Green Fluorescent Nissl Stain 495 524
Nile blue-EtOH 631 660
Nile red-phospolipid 553 637
Oregon Green™ 488 498 526
Oregon Green™ 514 513 532
PerCP 490 677
PicoGreen® 502 522
POPO-1 433 457
PO-PRO-1 433 456
Propidium Iodide (PI) 305 617
Qdot® 525 Nanocrystals 300 525
Qdot® 545 Nanocrystals 300 543
Qdot® 605 Nanocrystals 300 602
Qdot® 625 Nanocrystals 300 621
Qdot® 655 Nanocrystals 300 654
Qdot® 705 Nanocrystals 300 702
Qdot® 800 Nanocrystals 300 792
ReAsH-CCPGCC 592 606
Red 580 580 603
Rhodamine 110 497 519
Rhodamine 123 507 529
Rhodamine Green 497 523
Rhodamine Red-X 573 591
Rhodol Green 497 524
SNARF (carboxy) 514 Excitation pH9 576 638
SNARF-1 488nm (ph 9.0) 576 638
SNARF-1 514nm (ph 9.0) 576 638
Sodium Green 507 532
SpectrumAqua 434 481
SpectrumGreen 497 538
SpectrumRed 587 615
Sulforhodamine 101-EtOH 578 593
SYBR Gold nucleic acid gel stain-DNA 496 539
SYBR Green I nucleic acid gel stain-DNA 498 522
SYBR Safe DNA gel stain-DNA 509 526
SYTO 11 506 525
SYTO 17 619 638
SYTO 45 451 485
SYTO 61 618 650
SYTO RNASelect geen fluorescent cell stain 503 527
SYTOX Blue 444 470
SYTOX Green-DNA 504 524
TagCFP 458 480
Texas Red dextran 592 613
Texas Red DHPE 584 608
Texas Red-X® 595 613
TO-PRO-1 515 531
TOTO-1 514 531
TurboFP635(Katushka) 591 638
TurboYFP 525 538
Venus 516 528
VIC 530 550
Vybrant DyeCycle Green 506 534
Vybrant DyeCycle Orange 519 563
X-Rhod-1 Indicator 580 601
YFP (yellow GFP) 513 530
YO-PRO-3 613 629
YOYO-3 611 631
ZsYellow1 529 539

NTE Holder/Cube

This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm3.5 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.1 mm
(Can accept 2 mm dichroic)