This filter set is ideal for use with Green Fluorescent Protein (GFP) and other similar fluorophores. This hard-coated filter set is the best value on the market, delivering high brightness and contrast.
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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1,324.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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FF01-469/35-25
FL-004536 / 0
- Tavg > 90% 451.5 – 486.5 nm
- Center Wavelength of 469 nm and GMBW of 35 nm
- FWHM of 39.5 nm
FF01-525/39-25
FL-004547 / 0
- Tavg > 90% 505.5 – 544.5 nm
- Center Wavelength of 525 nm and GMBW of 39 nm
- FWHM of 44.9 nm
FF497-Di01-25x36
FL-007093 / 0
- Dichroic Beamsplitter with edge at 497 nm
- Ravg > 90% 452 – 490 nm
- Tavg > 90% 505 – 800 nm
- Flatness / RWE: Standard Epi-fluorescence
Custom sizing delivery confirmed ARO (sizing fee applies).
1 of 1
This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm The Nikon “true” cube needs a Di with corner chamfered (-QF) | 1.1 mm (Can accept a 2 mm dichroic) |
| Topic | Description | |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters | |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters | |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD | |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? |
|
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 5-FAM (5-carboxyfluorescein) | 492 | 518 |
|
|
| Acridine Orange (+DNA) | 502 | 526 |
|
|
| Acridine Yellow | 470 | 550 |
|
|
| Alexa Fluor® 430 | 431 | 541 |
|
|
| Alexa Fluor® 488 | 499 | 520 |
|
|
| AmCyan1 | 458 | 489 |
|
|
| ATTO 465 | 453 | 507 |
|
|
| ATTO 488 | 500 | 525 |
|
|
| ATTO 495 | 495 | 527 |
|
|
| BCECF (pH 5.5) | 481 | 518 |
|
|
| BCECF (pH 9.0) | 502 | 528 |
|
|
| BOBO™-1 | 461 | 484 |
|
|
| BODIPY FL | 505 | 512 |
|
|
| Calcein | 494 | 514 |
|
|
| Cerulean | 434 | 473 |
|
|
| CFP (ECFP) | 433 | 475 |
|
|
| CFP2 | 490 | 510 |
|
|
| Cy2™ | 492 | 507 |
|
|
| CyQUANT GR-DNA | 502 | 523 |
|
|
| DAF-FM-NO | 495 | 519 |
|
|
| Dendra2 (Green) | 491 | 507 |
|
|
| DiA | 457 | 586 |
|
|
| DiO | 475 | 500 |
|
|
| Dronpa | 502 | 516 |
|
|
| ecliptic pHluorin pH5.5 | 473 | 507 |
|
|
| Emerald | 491 | 511 |
|
|
| evoglow-Bs1 | 449 | 496 |
|
|
| FITC (Fluorescein) | 495 | 519 |
|
|
| Fluo-4 | 494 | 516 |
|
|
| Fluorescein dextran | 501 | 524 |
|
|
| Fluorescein-pH 8.0 | 489 | 517 |
|
|
| Fluoro-Emerald | 494 | 518 |
|
|
| FluoSpheres Yellow-Green fluorescent microspheres | 503 | 514 |
|
|
| FM 1-43 | 473 | 579 |
|
|
| GFP (EGFP) | 489 | 511 |
|
|
| Green 496 | 496 | 520 |
|
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| Green 500 | 501 | 524 |
|
|
| HCS LipidTOX Green phospholipidosis | 504 | 536 |
|
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| HiLyte Fluor™ 488 | 497 | 526 |
|
|
| LIVE-DEAD Fixable Green Dead Cell Stain | 498 | 525 |
|
|
| Lucifer yellow | 428 | 544 |
|
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| LysoSensor Green | 448 | 502 |
|
|
| LysoTracker Green | 501 | 509 |
|
|
| LysoTracker Yellow HCK-123 | 488 | 565 |
|
|
| mHoneyDew | 478 | 562 |
|
|
| MitoTracker™ Green | 490 | 512 |
|
|
| mTFP1 | 462 | 492 |
|
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| mWasabi | 493 | 509 |
|
|
| NBD-X (MeOH) | 467 | 538 |
|
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| NeuroTrace 500/525 Green Fluorescent Nissl Stain | 495 | 524 |
|
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| Oregon Green™ 488 | 498 | 526 |
|
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| PicoGreen® | 502 | 522 |
|
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| PKH67 | 488 | 500 |
|
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| Qdot® 525 Nanocrystals | 300 | 525 |
|
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| Qdot® 545 Nanocrystals | 300 | 543 |
|
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| Rhodamine 110 | 497 | 519 |
|
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| Rhodamine Green | 497 | 523 |
|
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| Rhodol Green | 497 | 524 |
|
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| sgGFP™ (super glow GFP) | 472 | 506 |
|
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| Sodium Green | 507 | 532 |
|
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| SpectrumGreen | 497 | 538 |
|
|
| SYBR Gold nucleic acid gel stain-DNA | 496 | 539 |
|
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| SYBR Green I nucleic acid gel stain-DNA | 498 | 522 |
|
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| SYTO 13 | 448 | 506 |
|
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| SYTO 16 | 489 | 520 |
|
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| SYTO 45 | 451 | 485 |
|
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| SYTO 9 | 483 | 500 |
|
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| SYTO RNASelect geen fluorescent cell stain | 503 | 527 |
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| SYTOX Green-DNA | 504 | 524 |
|
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| TagCFP | 458 | 480 |
|
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| TOTO-1 | 514 | 531 |
|
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| TurboGFP | 482 | 503 |
|
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| Vybrant DyeCycle Green | 506 | 534 |
|
|
| wtGFP (wild type GFP, non-UV excitation) | 474 | 509 |
|
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| YO-PRO-1 | 491 | 506 |
|
|
| YOYO-1 | 491 | 508 |
|
total selected
Clear selection
Select all 3 products from all pages.
FF01-469/35-25
FL-004536 / 0
- Tavg > 90% 451.5 – 486.5 nm
- Center Wavelength of 469 nm and GMBW of 35 nm
- FWHM of 39.5 nm
FF01-525/39-25
FL-004547 / 0
- Tavg > 90% 505.5 – 544.5 nm
- Center Wavelength of 525 nm and GMBW of 39 nm
- FWHM of 44.9 nm
FF497-Di01-25x36
FL-007093 / 0
- Dichroic Beamsplitter with edge at 497 nm
- Ravg > 90% 452 – 490 nm
- Tavg > 90% 505 – 800 nm
- Flatness / RWE: Standard Epi-fluorescence
Custom sizing delivery confirmed ARO (sizing fee applies).
1 of 1
This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm The Nikon “true” cube needs a Di with corner chamfered (-QF) | 1.1 mm (Can accept a 2 mm dichroic) |
| Topic | Description | |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters | |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters | |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD | |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? |
|
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