BrightLine® full-multiband filter set, optimized for FITC & Texas Red and other like fluorophores

Semrock Part Number: FITC/TxRed-A-NQF

IDEX Part Number: S-000205  /

Semrock

This new "full-multiband" filter set allows for simultaneous direct viewing or imaging with a color camera. Popular 2-fluorophore combinations include FITC/Texas Red and GFP/mCherry.

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  • Price Not Available.
  • 1,824.00 USD Each
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FF01-479/585-25

FL-004029 / 0

Out of Stock Backordered In Stock
  • Tavg > 90% 460 – 498 nm & Tavg > 90% 571.5 – 598.5 nm
  • Center Wavelengths of 479 nm & 585 nm and GMBWs of 38 nm & 27 nm

FF01-524/628-25

FL-004030 / 0

Out of Stock Backordered In Stock
  • Tavg > 90% 509.5 – 538.5 nm & Tavg > 90% 611.5 – 644.5 nm
  • Center Wavelengths of 524 nm & 628 nm and GMBWs of 29 nm & 33 nm

FF505/606-Di01-25x36

FL-006825 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edges at 505 nm & 606 nm
  • Ravg > 95% 458 – 499 nm & Ravg > 95% 570 – 600 nm
  • Tavg > 90% 509 – 541 nm & Tavg > 90% 612 – 647 nm

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This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm3.5 mm
Dichroic (L × W)25.2 mm × 35.6 mm

1.1 mm
(Can accept a 2 mm dichroic)
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
2-dodecylresorufin-lipid 582 595
5-carboxy-2,7-dichlorofluorescein 500 525
5-FAM (5-carboxyfluorescein) 492 518
5-ROX (carboxy-X-rhodamine) 578 604
7-AAD (7-aminoactinomycin D) 548 648
Acridine Orange (+DNA) 502 526
Acridine Yellow 470 550
Alexa Fluor® 430 431 541
Alexa Fluor® 488 499 520
Alexa Fluor® 500 503 525
Alexa Fluor® 568 579 603
Alexa Fluor® 594 590 618
AmCyan1 458 489
AsRed 2 576 592
ATTO 465 453 507
ATTO 488 500 525
ATTO 495 495 527
ATTO 590 594 624
ATTO 594 601 624
ATTO 610 614 634
ATTO 620 618 642
AzamiGreen 494 505
BCECF (pH 5.5) 481 518
BCECF (pH 9.0) 502 528
BOBO™-1 461 484
BOBO™-3 570 605
BODIPY 630/650-X 625 641
BODIPY FL 505 512
BODIPY FL-MeOH 502 511
BODIPY TR-X phallacidin 590 621
Calcein 494 514
Calcium Crimson 589 609
Calcium Green-1 507 529
CellTrace BODIPY TR methyl ester 598 625
CellTracker Red CMTPX 586 614
CFP2 490 510
Cy2™ 492 507
Cy3.5™ 578 591
CyQUANT GR-DNA 502 523
DAF-FM-NO 495 519
Dendra2 (Green) 491 507
DiO 475 500
Dronpa 502 516
DY-505-Phalloidin 503 530
DY-590 582 599
DyLight 594 593 618
ecliptic pHluorin pH5.5 473 507
Emerald 491 511
FITC (Fluorescein) 495 519
FlAsH-CCPFCC 511 530
Fluo-3 506 527
Fluo-4 494 516
Fluorescein dextran 501 524
Fluorescein-pH 8.0 489 517
Fluoro-Emerald 494 518
FluoSpheres Crimson fluorescent microspheres 620 646
FluoSpheres Red fluorescent microspheres 577 607
FluoSpheres Yellow-Green fluorescent microspheres 503 514
GFP (EGFP) 489 511
Green 496 496 520
Green 500 501 524
HcRed1 588 618
HCS CellMask Red cytoplasmic 622 644
HCS LipidTOX Green neutral lipid stain 498 507
HCS LipidTOX Green phospholipidosis 504 536
HCS LipidTOX Red neutral lipid stain 582 616
HCS LipidTOX Red phospholipidosis 584 608
HiLyte Fluor™ 488 497 526
HiLyte Fluor™ 594 592 616
Hypericin 600 603
KFP-Red 575 599
LIVE-DEAD Fixable Green Dead Cell Stain 498 525
LIVE-DEAD Fixable Red Dead Cell Stain 595 613
LysoSensor Green 448 502
LysoTracker Green 501 509
LysoTracker Red 573 592
LysoTracker Yellow HCK-123 488 565
Magnesium Green 507 531
mApple 566 594
mCherry 587 610
mHoneyDew 478 562
MitoTracker™ Green 490 512
MitoTracker™ Red 578 598
mKate2 588 633
mPlum 589 649
mRFP1 584 700
mRuby 558 605
mStrawberry 574 596
mTangerine 568 585
mTFP1 462 492
mWasabi 493 509
NBD-X (MeOH) 467 538
NeuroTrace 500/525 Green Fluorescent Nissl Stain 495 524
Nile red-phospolipid 553 637
Oregon Green™ 488 498 526
Oregon Green™ 514 513 532
PicoGreen® 502 522
PKH67 488 500
Qdot® 525 Nanocrystals 300 525
Qdot® 605 Nanocrystals 300 602
Qdot® 625 Nanocrystals 300 621
ratiometric pHluorin pH5 500 509
ReAsH-CCPGCC 592 606
Red 580 580 603
Rhodamine 110 497 519
Rhodamine 123 507 529
Rhodamine Green 497 523
Rhodamine Red-X 573 591
Rhodol Green 497 524
rsGFP (red shifted GFP, S65T) 498 516
sgGFP™ (super glow GFP) 472 506
SNARF (carboxy) 514 Excitation pH9 576 638
SNARF-1 488nm (ph 9.0) 576 638
SNARF-1 514nm (ph 9.0) 576 638
Sodium Green 507 532
SpectrumGreen 497 538
SpectrumRed 587 615
Sulforhodamine 101-EtOH 578 593
SYBR Gold nucleic acid gel stain-DNA 496 539
SYBR Green I nucleic acid gel stain-DNA 498 522
SYBR Safe DNA gel stain-DNA 509 526
SYTO 11 506 525
SYTO 13 448 506
SYTO 16 489 520
SYTO 17 619 638
SYTO 59 622 644
SYTO 61 618 650
SYTO 9 483 500
SYTO RNASelect geen fluorescent cell stain 503 527
SYTOX Green-DNA 504 524
TagCFP 458 480
Texas Red dextran 592 613
Texas Red DHPE 584 608
Texas Red® 592 614
TO-PRO-1 515 531
TOTO-1 514 531
TurboFP635(Katushka) 591 638
TurboGFP 482 503
Vybrant DyeCycle Green 506 534
wtGFP (wild type GFP, non-UV excitation) 474 509
X-Rhod-1 Indicator 580 601
YO-PRO-1 491 506
YO-PRO-3 613 629
YOYO-1 491 508
YOYO-3 611 631

Related Products

FF01-479/585-25

FL-004029 / 0

Out of Stock Backordered In Stock
  • Tavg > 90% 460 – 498 nm & Tavg > 90% 571.5 – 598.5 nm
  • Center Wavelengths of 479 nm & 585 nm and GMBWs of 38 nm & 27 nm

FF01-524/628-25

FL-004030 / 0

Out of Stock Backordered In Stock
  • Tavg > 90% 509.5 – 538.5 nm & Tavg > 90% 611.5 – 644.5 nm
  • Center Wavelengths of 524 nm & 628 nm and GMBWs of 29 nm & 33 nm