BrightLine® full-multiband filter set, optimized for DAPI & FITC and other like fluorophores

Semrock Part Number: DA/FI-A-000

IDEX Part Number: S-000726  /

Semrock

This "full-multiband" filter set allows for simultaneous direct viewing or imaging of DAPI and FITC with a color camera.

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  • Price Not Available.
  • 1,415.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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Filter set mounted in microscope holders/cubes found below in related products section.
Set Components

FF01-387/480-25

FL-004187

Each
  • Tavg > 80% 381.5 – 392.5 nm & Tavg > 90% 466 – 495 nm
  • Center Wavelengths of 387 nm & 480 nm and GMBWs of 11 nm & 29 nm

FL-004187

FF01-433/530-25

FL-004229

Each
  • Tavg > 90% 414 – 452 nm & Tavg > 90% 510 – 550 nm
  • Center Wavelengths of 433 nm & 530 nm and GMBWs of 38 nm & 40 nm

FL-004229

FF403/502-Di01-25x36

FL-006945

Each
  • Dichroic Beamsplitter with edges at 403 nm & 502 nm
  • Ravg > 97.5% 370 – 393 nm & Ravg > 97.5% 466 – 495 nm
  • Tavg > 90% 414 – 452 nm & Tavg > 90% 510 – 550 nm

FL-006945

Optical Specifications Value
Blocking BrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.
General Filter Specifications Value
Optical Damage Rating Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.
Microscope Compatibility BrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Physical Filter Specifications   Value
Scratch-Dig 60-40
Dichroic Orientation Reflective coating side should face toward light source.

DA/FI-A-NQF

S-000158 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series

DA/FI-A-NTE

S-001334 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator

DA/FI-A-OMF

S-001335 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81

DA/FI-A-ZHE

S-001336 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1

DA/FI-A-OFF

S-000159 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-FF for microscopes: BX53, BX63

DA/FI-A-LDMK

S-001332 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Leica DM-K for microscopes: DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, DM-6000, DMI6000 B, DM4 and DM6

DA/FI-A-LDMP

S-001333 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Leica DMi8 (P-Cube) for microscopes: DMi8

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TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
1,8-ANS 372 477
4-methylumbelliferone 357 441
5-carboxy-2,7-dichlorofluorescein 500 525
5-FAM (5-carboxyfluorescein) 492 518
6-carboxyrhodamine 6G 525 555
7-hydroxy-4-methylcoumarin 360 442
Acridine Orange (+DNA) 502 526
Acridine Yellow 470 550
Alexa Fluor® 405 401 422
Alexa Fluor® 430 431 541
Alexa Fluor® 488 499 520
Alexa Fluor® 500 503 525
AMCA (Aminomethylcoumarin) 350 448
AmCyan1 458 489
ATTO 488 500 525
ATTO 495 495 527