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BrightLine® full-multiband filter set, optimized for DAPI & FITC and other like fluorophores

Semrock Part Number: DA/FI-A-000

IDEX Part Number: S-000726  /

Semrock

This "full-multiband" filter set allows for simultaneous direct viewing or imaging of DAPI and FITC with a color camera.

  • Price Not Available.
  • Each
    US Domestic pricing – contact your local distributor for other currency list prices
  • Price Not Available.
  • 1,415.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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Filter set mounted in microscope holders/cubes found below in related products section.
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Set Components

FF01-387/480-25

FL-004187

Each
  • Tavg > 80% 381.5 – 392.5 nm & Tavg > 90% 466 – 495 nm
  • Center Wavelengths of 387 nm & 480 nm and GMBWs of 11 nm & 29 nm

FL-004187

FF01-433/530-25

FL-004229

Each
  • Tavg > 90% 414 – 452 nm & Tavg > 90% 510 – 550 nm
  • Center Wavelengths of 433 nm & 530 nm and GMBWs of 38 nm & 40 nm

FL-004229

FF403/502-Di01-25x36

FL-006945

Each
  • Dichroic Beamsplitter with edges at 403 nm & 502 nm
  • Ravg > 97.5% 370 – 393 nm & Ravg > 97.5% 466 – 495 nm
  • Tavg > 90% 414 – 452 nm & Tavg > 90% 510 – 550 nm

FL-006945

DA/FI-A-NQF

S-000158 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series

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DA/FI-A-NTE

S-001334 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator

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DA/FI-A-OMF

S-001335 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81

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DA/FI-A-ZHE

S-001336 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1

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DA/FI-A-OFF

S-000159 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-FF for microscopes: BX53, BX63

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DA/FI-A-LDMK

S-001332 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Leica DM-K for microscopes: DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, DM-6000, DMI6000 B, DM4 and DM6

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DA/FI-A-LDMP

S-001333 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Leica DMi8 (P-Cube) for microscopes: DMi8

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Blocking
BrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

Microscope Compatibility
BrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.

Optical Damage Rating
Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.

Scratch-Dig
60-40

Exciter/Emitter Orientation
Arrow on ring indicates preferred direction of propagation of light.

Dichroic Orientation
Reflective coating side should face toward light source and sample.

Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
1,8-ANS 372 477
4-methylumbelliferone 357 441
5-carboxy-2,7-dichlorofluorescein 500 525
5-FAM (5-carboxyfluorescein) 492 518
6-carboxyrhodamine 6G 525 555
7-hydroxy-4-methylcoumarin 360 442
Acridine Orange (+DNA) 502 526
Acridine Yellow 470 550
Alexa Fluor® 405 401 422
Alexa Fluor® 430 431 541
Alexa Fluor® 488 499 520
Alexa Fluor® 500 503 525
AMCA (Aminomethylcoumarin) 350 448
AmCyan1 458 489
ATTO 488 500 525
ATTO 495 495 527
BCECF (pH 5.5) 481 518
BCECF (pH 9.0) 502 528
BD Horizon V450 406 449
BFP (EBFP) 380 440
BOBO™-1 461 484
BODIPY FL 505 512
BODIPY FL-MeOH 502 511
Calcein 494 514
Calcium Green-1 507 529
Calcofluor White 351 444
Cascade Blue™ 401 419
CellTrace calcein violet 400 452
CFP2 490 510
Cy2™ 492 507
CyQUANT GR-DNA 502 523
DAF-FM-NO 495 519
DAPI 359 461
Dendra2 (Green) 491 507
DiA 457 586
DiFMU-pH 9.0 358 450
DiO 475 500
Dronpa 502 516
DY-505-Phalloidin 503 530
DyLight 405 398 420
ecliptic pHluorin pH5.5 473 507
Emerald 491 511
FITC (Fluorescein) 495 519
FlAsH-CCPFCC 511 530
Fluo-3 506 527
Fluo-4 494 516
Fluorescein dextran 501 524
Fluorescein-pH 8.0