A special long-pass emission filter in this filter set allows simultaneous viewing by eye or with a color camera of samples that are multi-labeled with quantum dot nanocrystals. It takes advantage of the unique fact that all quantum dots share a common UV/blue excitation spectrum while simultaneously exhibiting narrow and specific visible emission spectra. The highly transmitting, deep-blue exciter efficiently excites all quantum dots with no UV light to damage samples or generate undesirable background, while the high and uniform transmission of the long-pass emission filter provides exceptional and balanced brightness. And with the "no burn-out" performance shared by all BrightLine filters, the permanent performance of this set will outlast even your quantum dots!
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This set is mounted in an Evident Scientific (Olympus) OFF Fluorescence Filter Holder, which is compatible with the BX3 (BX53, BX63) and IX3 Series (IX53, IX73, IX83) of Evident Scientific (Olympus) microscopes.
Filter Role | Filter Size | Maximum Thickness |
Exciter (Diameter) | 25.0 mm | 5.0 mm |
Emitter (Diameter) | 25.0 mm | 5.0 mm |
Dichroic (L × W) | 26 mm x 38 mm | 1.5 mm |
Topic | Description |
Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
FL-003926