This set is designed to take advantage of the superior resolution, sensitivity, and image fidelity of today's state-of-the-art laser-based microscopes. The filter spectra are optimized to capture the maximum fluorescence from Cy5 and other similar fluorophores while offering superb blocking of popular 635 nm (solid-state laser diode), 633 nm (HeNe gas) or 647 nm (Kr-ion gas) lasers. The dichroic offers excellent flatness for minimal focus shift and aberrations of the laser beam spot at the sample.
Our improved version is certified to deliver dichroic flatness <= 1λ P-V RWE @ 632.8 nm for loose and cube mounted [NTE, OFF, OMF or ZHE] filter sets LF635-C
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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1,355.03 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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0.000.00 -
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We now offer LFOV (large field of view) cube sets compatible with Nikon Eclipse Ti2 microscopes. Please contact us for more information.
Set Components
Di02-R635-25x36FL-007077
Each
|
FL-007077 |
|
FF01-640/14-25FL-004697
Each
|
FL-004697 |
|
FF01-676/29-25FL-004567
Each
|
FL-004567 |
total selected
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LF635-B-U03
S-000912 / 0
This set is delivered unmounted and fits Olympus OFF cubes which is compatible with the BX3 (BX53, BX63) and IX3 Series (IX53, IX73, IX83) of Evident Scientific (Olympus) microscopes.
LF635-C-000
S-000913 / 0
1λ P-V RWE super-resolution laser optimized single-band filter set. Ideal for use with: Cy5, Alexa Fluor® 647, APC, ATTO 647, BODIPY 650/665-X, MitoTracker™ Deep Red
LF635-B-NQF
S-001854 / 0
This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series
LF635-B-NTE
S-000495 / 0
This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator
LF635-B-OMF
S-000663 / 0
This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81
LF635-B-ZHE
S-001856 / 0
This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1
LF635-B-OFF
S-001855 / 0
This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-FF for microscopes: BX53, BX63
1 of 1
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
| Link | Description |
| Request a Drawing | Looking for an optical filter drawing file for this product? Request one now! |
| Request Custom Optical Filters | Contact us today to start a conversation about custom optical filters. |
| Access Optical Filter Warranty | Find our warranty in our Terms & Conditions of Sale. |
| Semrock Optical Filters RoHS Certificate | RoHS, stands for Restriction of Hazardous Substances. The current RoHS Directive currently restricts the use of ten substances. |
| Semrock Optical Filters REACH Certificate | REACH is a regulation of the European Union, adopted to improve the protection of human health and the environment from the risks that can be posed by chemicals, while enhancing the competitiveness of the EU chemicals industry. |
| Download Optical Filters 101 Guide | Find an in-depth overview of optical filter principles, specifications, and applications. Request a digital copy of this guide now. |
| Request Clean Filter Checklist Poster | Looking to clean your optical filters? Need instructions? Request a printed copy of our Clean Filter Checklist Poster. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 5-carboxynapthofluorescein (pH 10) | 555 | 615 |
|
|
| Alexa Fluor® 633 | 631 | 647 |
|
|
| Alexa Fluor® 635 | 633 | 648 |
|
|
| Alexa Fluor® 647 | 653 | 668 |
|
|
| Alexa Fluor® 660 | 663 | 691 |
|
|
| APC | 651 | 660 |
|
|
| APC (Allophycocyanin) | 651 | 660 |
|
|
| ATTO 633 | 629 | 658 |
|
|
| ATTO 635 | 635 | 658 |
|
|
| ATTO 647 | 644 | 670 |
|
|
| ATTO 647N | 645 | 668 |
|
|
| ATTO 655 | 663 | 683 |
|
|
| BODIPY 650/665-X | 646 | 664 |
|
|
| Carboxynaphthofluorescein | 599 | 674 |
|
|
| Cy5.5â„¢ | 672 | 690 |
|
|
| Cy5â„¢ | 649 | 666 |
|
|
| DDAO | 648 | 657 |
|
|
| DiD (DiIC18(5)) - Lipophilic Tracer | 648 | 670 |
|
|
| DY-630 | 634 | 656 |
|
|
| DyLight 633 | 620 | 649 |
|
|
| DyLight 649 | 651 | 673 |
|
|
| FluoSpheres Crimson fluorescent microspheres | 620 | 646 |
|
|
| FluoSpheres Dark Red fluorescent microspheres | 656 | 683 |
|
|
| HCS CellMask Red cytoplasmic | 622 | 644 |
|
|
| HCS LipidTOX Deep Red neutral lipid stain | 635 | 652 |
|
|
| HiLyte Fluorâ„¢ 647 | 649 | 673 |
|
|
| LIVE-DEAD Fixable Far Red Dead Cell Stain | 653 | 668 |
|
|
| MitoTrackerâ„¢ Deep Red | 641 | 662 |
|
|
| Nile blue-EtOH | 631 | 660 |
|
|
| Qdot® 705 Nanocrystals | 300 | 702 |
|
|
| Red 650 | 649 | 665 |
|
|
| SpectrumFRed (Far Red) | 650 | 673 |
|
|
| SYTO 59 | 622 | 644 |
|
|
| SYTO 60 | 650 | 681 |
|
|
| SYTO 61 | 618 | 650 |
|
|
| SYTOX Red-DNA | 640 | 658 |
|
|
| TO-PRO-3 | 642 | 657 |
|
|
| TOTO-3 | 642 | 661 |
|
total selected
Clear selection
Select all 7 products from all pages.
LF635-B-U03
S-000912 / 0
This set is delivered unmounted and fits Olympus OFF cubes which is compatible with the BX3 (BX53, BX63) and IX3 Series (IX53, IX73, IX83) of Evident Scientific (Olympus) microscopes.
LF635-C-000
S-000913 / 0
1λ P-V RWE super-resolution laser optimized single-band filter set. Ideal for use with: Cy5, Alexa Fluor® 647, APC, ATTO 647, BODIPY 650/665-X, MitoTracker™ Deep Red
LF635-B-NQF
S-001854 / 0
This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series
LF635-B-NTE
S-000495 / 0
This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator
LF635-B-OMF
S-000663 / 0
This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81
LF635-B-ZHE
S-001856 / 0
This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1
LF635-B-OFF
S-001855 / 0
This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-FF for microscopes: BX53, BX63
1 of 1
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
| Link | Description |
| Request a Drawing | Looking for an optical filter drawing file for this product? Request one now! |
| Request Custom Optical Filters | Contact us today to start a conversation about custom optical filters. |
| Access Optical Filter Warranty | Find our warranty in our Terms & Conditions of Sale. |
| Semrock Optical Filters RoHS Certificate | RoHS, stands for Restriction of Hazardous Substances. The current RoHS Directive currently restricts the use of ten substances. |
| Semrock Optical Filters REACH Certificate | REACH is a regulation of the European Union, adopted to improve the protection of human health and the environment from the risks that can be posed by chemicals, while enhancing the competitiveness of the EU chemicals industry. |
| Download Optical Filters 101 Guide | Find an in-depth overview of optical filter principles, specifications, and applications. Request a digital copy of this guide now. |
| Request Clean Filter Checklist Poster | Looking to clean your optical filters? Need instructions? Request a printed copy of our Clean Filter Checklist Poster. |
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