This set is designed to take advantage of the superior resolution, sensitivity, and image fidelity of today's state-of-the-art laser-based microscopes. The filter spectra are optimized to capture the maximum fluorescence from CFP and other similar fluorophores while offering superb blocking of popular 442 nm (solid-state) lasers. The dichroic offers excellent flatness for minimal focus shift and aberrations of the laser beam spot at the sample.
Our improved version is certified to deliver dichroic flatness <=1 λ P-V RWE @ 632.8 nm for loose and cube mounted [NTE, OFF, OMF or ZHE] filter sets LF442-C
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LF442-C-000
S-000890 / 0
1λ P-V RWE super-resolution laser optimized single-band filter set. Ideal for use with: CFP, Cerulean, TagCFP, mTFP1, Brilliant™ Violet 480, ATTO 425
LF442-B-NQF
S-001772 / 0
This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series
LF442-B-NTE
S-000456 / 0
This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator
LF442-B-OMF
S-001774 / 0
This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81
LF442-B-ZHE
S-001775 / 0
This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1
LF442-B-OFF
S-001773 / 0
This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-FF for microscopes: BX53, BX63
1 of 1
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| Acridine Yellow | 470 | 550 |
|
|
| AmCyan1 | 458 | 489 |
|
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| Aqua 431 | 430 | 478 |
|
|
| ATTO 425 | 436 | 484 |
|
|
| ATTO 465 | 453 | 507 |
|
|
| BD Horizon V500 | 410 | 500 |
|
|
| BOBOâ„¢-1 | 461 | 484 |
|
|
| Cerulean | 434 | 473 |
|
|
| CFP (ECFP) | 433 | 475 |
|
|
| DEAC | 432 | 472 |
|
|
| ECFP | 435 | 475 |
|
|
| evoglow-Bs1 | 449 | 496 |
|
|
| LysoSensor Green | 448 | 502 |
|
|
| mTFP1 | 462 | 492 |
|
|
| POPO-1 | 433 | 457 |
|
|
| PO-PRO-1 | 433 | 456 |
|
|
| SpectrumAqua | 434 | 481 |
|
|
| SYTO 45 | 451 | 485 |
|
|
| SYTOX Blue | 444 | 470 |
|
|
| TagCFP | 458 | 480 |
|
total selected
Clear selection
Select all 6 products from all pages.
LF442-C-000
S-000890 / 0
1λ P-V RWE super-resolution laser optimized single-band filter set. Ideal for use with: CFP, Cerulean, TagCFP, mTFP1, Brilliant™ Violet 480, ATTO 425
LF442-B-NQF
S-001772 / 0
This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series
LF442-B-NTE
S-000456 / 0
This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator
LF442-B-OMF
S-001774 / 0
This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81
LF442-B-ZHE
S-001775 / 0
This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1
LF442-B-OFF
S-001773 / 0
This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-FF for microscopes: BX53, BX63
1 of 1
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
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