This "Sedat" multiband set is optimized for laser excitation over four bands - utilizing a 375 or 405 nm laser source for DAPI, a 473 or 488 nm laser source for GFP or FITC, a 559, 561.4 or 568.2 nm laser source for mCherry, and a 632.8, 635, or 647.1 nm laser source for Cy5. This set provides high brightness, extremely low crosstalk, and superb signal-to-noise ratio. The dichroic offers industry-leading 1λ P-V RWE for minimal focus shift and aberrations of the laser beam spot up to 10 mm diameter to enable popular imaging and Super-Resolution techniques including TIRF, PALM, STORM, and Structured-Illumination.
Loose and cube mounted filter sets are certified to deliver dichroic flatness <= 1λ P-V RWE @ 632.8 nm.
Looking for this filter set in one of the following cubes: NQF, LDMK or LDMP?
Our previous version is still available for those cube configurations LF405/488/561/635-4X4M-A
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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3,939.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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0.000.00 -
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- Notify me when available
Filter set mounted in microscope holders/cubes found below in related products section.
Set Components
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FL-007139 |
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FL-004666 |
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FL-004531 |
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FL-004450 |
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FL-004686 |
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FL-004393 |
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FL-004697 |
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FL-004567 |
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FL-004712 |
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1 of 1
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 1,8-ANS | 372 | 477 |
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| 2-dodecylresorufin-lipid | 582 | 595 |
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| 4-methylumbelliferone | 357 | 441 |
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| 5-carboxy-2,7-dichlorofluorescein | 500 | 525 |
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| 5-carboxynapthofluorescein (pH 10) | 555 | 615 |
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| 5-FAM (5-carboxyfluorescein) | 492 | 518 |
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| 5-ROX (carboxy-X-rhodamine) | 578 | 604 |
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| 5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0) | 542 | 568 |
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| 7-AAD (7-aminoactinomycin D) | 548 | 648 |
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| 7-hydroxy-4-methylcoumarin | 360 | 442 |
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| Acridine Orange (+DNA) | 502 | 526 |
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| Alexa Fluor® 405 | 401 | 422 |
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| Alexa Fluor® 430 | 431 | 541 |
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| Alexa Fluor® 488 | 499 | 520 |
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| Alexa Fluor® 500 | 503 | 525 |
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| Alexa Fluor® 568 | 579 | 603 |
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| Alexa Fluor® 633 | 631 | 647 |
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| Alexa Fluor® 635 | 633 | 648 |
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| Alexa Fluor® 647 | 653 | 668 |
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| Alexa Fluor® 660 | 663 | 691 |
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| Alexa Fluor® 680 | 679 | 702 |
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| AMCA (Aminomethylcoumarin) | 350 | 448 |
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| AmCyan1 | 458 | 489 |
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| Amplex UltraRed peroxidation product (pH 7.5) | 568 | 581 |
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| APC | 651 | 660 |
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| APC (Allophycocyanin) | 651 | 660 |
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| AsRed 2 | 576 | 592 |
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| ATTO 488 | 500 | 525 |
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| ATTO 495 | 495 | 527 |
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| ATTO 565 | 563 | 592 |
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| ATTO 633 | 629 | 658 |
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| ATTO 635 | 635 | 658 |
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| ATTO 647 | 644 | 670 |
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| ATTO 647N | 645 | 668 |
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| ATTO 655 | 663 | 683 |
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| ATTO 680 | 680 | 700 |
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| BCECF (pH 5.5) | 481 | 518 |
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| BCECF (pH 9.0) | 502 | 528 |
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| BD Horizon V450 | 406 | 449 |
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| BFP (EBFP) | 380 | 440 |
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| BOBO™-3 | 570 | 605 |
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| BODIPY 630/650-X | 625 | 641 |
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| BODIPY 650/665-X | 646 | 664 |
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| BODIPY FL | 505 | 512 |
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| BODIPY FL-MeOH | 502 | 511 |
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| BODIPY TR-X phallacidin | 590 | 621 |
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| Calcein | 494 | 514 |
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| Calcium Crimson | 589 | 609 |
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| Calcium Green-1 | 507 | 529 |
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| Calcofluor White | 351 | 444 |
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| Carboxynaphthofluorescein | 599 | 674 |
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| Cascade Blue™ | 401 | 419 |
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| CellTrace calcein violet | 400 | 452 |
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| CellTracker Red CMTPX | 586 | 614 |
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| CFP2 | 490 | 510 |
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| Cy2™ | 492 | 507 |
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| Cy3.5™ | 578 | 591 |
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| Cy5.5™ | 672 | 690 |
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| Cy5™ | 649 | 666 |
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| CyQUANT GR-DNA | 502 | 523 |
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| DAF-FM-NO | 495 | 519 |
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| DAPI | 359 | 461 |
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| DDAO | 648 | 657 |
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| Dendra2 (Green) | 491 | 507 |
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| Dendra2 (Red) | 554 | 569 |
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| DiD (DiIC18(5)) - Lipophilic Tracer | 648 | 670 |
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| DiFMU-pH 9.0 | 358 | 450 |
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| DiO | 475 | 500 |
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| Dronpa | 502 | 516 |
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| DsRed | 559 | 583 |
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| DsRed-Express | 556 | 584 |
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| dTomato | 556 | 582 |
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| DY-505-Phalloidin | 503 | 530 |
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| DY-590 | 582 | 599 |
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| DY-630 | 634 | 656 |
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| DY-675 | 675 | 699 |
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| DyLight 405 | 398 | 420 |
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| DyLight 633 | 620 | 649 |
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| DyLight 649 | 651 | 673 |
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| DyLight 680 | 676 | 705 |
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| ecliptic pHluorin pH5.5 | 473 | 507 |
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| Emerald | 491 | 511 |
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| ER-Tracker™ Blue-White DPX | 372 | 557 |
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| Ethidium bromide | 518 | 603 |
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| Ethidium homodimer | 527 | 617 |
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| FITC (Fluorescein) | 495 | 519 |
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| FlAsH-CCPFCC | 511 | 530 |
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| Fluo-3 | 506 | 527 |
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| Fluo-4 | 494 | 516 |
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| Fluorescein dextran | 501 | 524 |
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| Fluorescein-pH 8.0 | 489 | 517 |
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| Fluoro-Emerald | 494 | 518 |
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| FluoSpheres Crimson fluorescent microspheres | 620 | 646 |
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| FluoSpheres Dark Red fluorescent microspheres | 656 | 683 |
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| FluoSpheres Red fluorescent microspheres | 577 | 607 |
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| FluoSpheres Yellow-Green fluorescent microspheres | 503 | 514 |
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| GFP (EGFP) | 489 | 511 |
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| Green 496 | 496 | 520 |
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| Green 500 | 501 | 524 |
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| HCS CellMask Red cytoplasmic | 622 | 644 |
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| HCS LipidTOX Deep Red neutral lipid stain | 635 | 652 |
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| HCS LipidTOX Green neutral lipid stain | 498 | 507 |
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| HCS LipidTOX Green phospholipidosis | 504 | 536 |
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| HCS LipidTOX Red neutral lipid stain | 582 | 616 |
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| HCS LipidTOX Red phospholipidosis | 584 | 608 |
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| HiLyte Fluor™ 405 | 403 | 428 |
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| HiLyte Fluor™ 488 | 497 | 526 |
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| HiLyte Fluor™ 647 | 649 | 673 |
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| HiLyte Fluor™ 680 | 688 | 702 |
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| Hoechst 33258 | 352 | 455 |
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| Hypericin | 600 | 603 |
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| IRDye 680 NHS Ester | 683 | 710 |
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| IRDye 700Phosphoramidite | 691 | 699 |
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| KFP-Red | 575 | 599 |
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| LDS 751 | 560 | 711 |
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| LIVE-DEAD Fixable Far Red Dead Cell Stain | 653 | 668 |
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| LIVE-DEAD Fixable Green Dead Cell Stain | 498 | 525 |
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| LIVE-DEAD Fixable Violet Dead Cell Stain | 403 | 455 |
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| LOLO-1 | 568 | 580 |
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| LysoSensor Blue | 374 | 424 |
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| LysoSensor Green | 448 | 502 |
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| LysoTracker Blue | 373 | 422 |
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| LysoTracker Green | 501 | 509 |
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| LysoTracker Red | 573 | 592 |
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| LysoTracker Yellow HCK-123 | 488 | 565 |
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| Magnesium Green | 507 | 531 |
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| mApple | 566 | 594 |
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| Marina Blue | 363 | 461 |
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| mCherry | 587 | 610 |
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| Merocyanine 540 | 559 | 579 |
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| mHoneyDew | 478 | 562 |
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| MitoTracker™ Deep Red | 641 | 662 |
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| MitoTracker™ Green | 490 | 512 |
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| MitoTracker™ Red | 578 | 598 |
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| mKate2 | 588 | 633 |
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| mRFP | 555 | 583 |
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| mRFP1 | 584 | 700 |
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| mRuby | 558 | 605 |
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| mStrawberry | 574 | 596 |
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| mTangerine | 568 | 585 |
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| mWasabi | 493 | 509 |
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| NBD-X (MeOH) | 467 | 538 |
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| NeuroTrace 500/525 Green Fluorescent Nissl Stain | 495 | 524 |
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| Nile blue-EtOH | 631 | 660 |
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| Nile red-phospolipid | 553 | 637 |
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| Oregon Green™ 488 | 498 | 526 |
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| Oregon Green™ 514 | 513 | 532 |
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| Pacific Blue™ | 404 | 455 |
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| pHrodo™, succinimidyl ester | 560 | 587 |
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| PicoGreen® | 502 | 522 |
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| PKH67 | 488 | 500 |
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| Propidium Iodide (PI) | 305 | 617 |
|
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| Pro-Q Diamond | 556 | 583 |
|
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| Qdot® 525 Nanocrystals | 300 | 525 |
|
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| Qdot® 585 Nanocrystals | 300 | 588 |
|
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| Qdot® 605 Nanocrystals | 300 | 602 |
|
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| Qdot® 655 Nanocrystals | 300 | 654 |
|
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| Qdot® 705 Nanocrystals | 300 | 702 |
|
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| Quasar® 705 | 688 | 706 |
|
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| ratiometric pHluorin pH5 | 500 | 509 |
|
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| ReAsH-CCPGCC | 592 | 606 |
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| Red 580 | 580 | 603 |
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| Red 650 | 649 | 665 |
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| Resorufin | 571 | 584 |
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| Rhodamine 110 | 497 | 519 |
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| Rhodamine 123 | 507 | 529 |
|
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| Rhodamine Green | 497 | 523 |
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| Rhodamine phalloidin | 558 | 575 |
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| Rhodamine Red-X | 573 | 591 |
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| Rhodol Green | 497 | 524 |
|
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| sgBFP™ | 387 | 451 |
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| sgGFP™ (super glow GFP) | 472 | 506 |
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| Sirius | 355 | 424 |
|
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| SNARF (carboxy) 514 Excitation pH9 | 576 | 638 |
|
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| SNARF-1 488nm (ph 6.0) | 548 | 586 |
|
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| SNARF-1 488nm (ph 9.0) | 576 | 638 |
|
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| SNARF-1 514nm (ph 6.0) | 549 | 587 |
|
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| SNARF-1 514nm (ph 9.0) | 576 | 638 |
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| Sodium Green | 507 | 532 |
|
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| SpectrumBlue | 405 | 449 |
|
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| SpectrumFRed (Far Red) | 650 | 673 |
|
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| SpectrumGreen | 497 | 538 |
|
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| SpectrumOrange | 554 | 587 |
|
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| Sulforhodamine 101-EtOH | 578 | 593 |
|
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| SYBR Gold nucleic acid gel stain-DNA | 496 | 539 |
|
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| SYBR Green I nucleic acid gel stain-DNA | 498 | 522 |
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| SYBR Safe DNA gel stain-DNA | 509 | 526 |
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| SYTO 11 | 506 | 525 |
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| SYTO 13 | 448 | 506 |
|
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| SYTO 16 | 489 | 520 |
|
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| SYTO 17 | 619 | 638 |
|
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| SYTO 59 | 622 | 644 |
|
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| SYTO 60 | 650 | 681 |
|
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| SYTO 61 | 618 | 650 |
|
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| SYTO 9 | 483 | 500 |
|
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| SYTO RNASelect geen fluorescent cell stain | 503 | 527 |
|
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| SYTOX Green-DNA | 504 | 524 |
|
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| SYTOX Red-DNA | 640 | 658 |
|
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| TagBFP | 402 | 457 |
|
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| Tetramethylrhodamine dextran | 554 | 582 |
|
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| Texas Red dextran | 592 | 613 |
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| Texas Red DHPE | 584 | 608 |
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| TO-PRO-1 | 515 | 531 |
|
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| TO-PRO-3 | 642 | 657 |
|
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| TOTO-1 | 514 | 531 |
|
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| TOTO-3 | 642 | 661 |
|
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| TRITC (Tetramethylrhodamine) | 552 | 578 |
|
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| TurboGFP | 482 | 503 |
|
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| TurboRFP | 553 | 573 |
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| Vybrant DyeCycle Green | 506 | 534 |
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| Vybrant DyeCycle Violet | 369 | 437 |
|
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| wtGFP (wild type GFP, non-UV excitation) | 474 | 509 |
|
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| X-Rhod-1 Indicator | 580 | 601 |
|
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| YO-PRO-1 | 491 | 506 |
|
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| YOYO-1 | 491 | 508 |
|
total selected
Clear selection
Select all 1 products from all pages.
1 of 1
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
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