BrightLine® FRET filter set, optimized for Cyan & Yellow Fluorescent Protein and other like fluorophores

Semrock Part Number: FRET-CFP/YFP-C-000

IDEX Part Number: S-000765  /

Semrock

This four-filter set is specially designed to achieve high-performance fast FRET (Fluorescence Resonance Energy Transfer) imaging for cyan donor fluorescence and yellow acceptor fluorescence. FRET measurements using wide-field microscopy are challenging – using the best possible filters can make a significant difference. These filter combinations provide excellent brightness and signal-to-noise ratio while minimizing the undesirable donor-acceptor crosstalk.

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  • 1,444.30 USD Each
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Filter set mounted in microscope holders/cubes found below in related products section.
Set Components

FF01-483/32-25

FL-004598

Each
  • Tavg > 93% 467 – 499 nm
  • Center Wavelength of 483 nm and GMBW of 32 nm
  • FWHM of 38.2 nm

FL-004598

FF01-542/27-25

FL-004609

Each
  • Tavg > 93% 528.5 – 555.5 nm
  • Center Wavelength of 542 nm and GMBW of 27 nm
  • FWHM of 33.1 nm

FL-004609

FF02-438/24-25

FL-004649

Each
  • Tavg > 93% 426 – 450 nm
  • Center Wavelength of 438 nm and GMBW of 24 nm
  • FWHM of 29.5 nm

FL-004649

FF458-Di02-25x36

FL-007114

Each
  • Dichroic Beamsplitter with edge at 458 nm
  • Ravg > 98% 350 – 450 nm
  • Tavg > 93% 467 – 950 nm
  •  Flatness / RWE: Standard Epi-fluorescence

Custom sizing delivery confirmed ARO (sizing fee applies).

FL-007114

Optical Specifications Value
Blocking BrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMakerâ„¢ fluorescence filter design.
General Filter Specifications Value
Optical Damage Rating Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.
Microscope Compatibility BrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Physical Filter Specifications   Value
Scratch-Dig 60-40
Dichroic Orientation Reflective coating side should face toward light source.

FRET-CFP/YFP-C-U03

S-000768 / 0

Out of Stock Backordered In Stock
This set is delivered unmounted and fits Olympus OFF cubes which is compatible with the BX3 (BX53, BX63) and IX3 Series (IX53, IX73, IX83) of Evident Scientific (Olympus) microscopes.

FRET-CFP/YFP-C-U03-ZERO

S-000769 / 0

Out of Stock Backordered In Stock
This set is delivered unmounted and fits Olympus OFF cubes which is compatible with the BX3 (BX53, BX63) and IX3 Series (IX53, IX73, IX83) of Evident Scientific (Olympus) microscopes.

FRET-CFP/YFP-C-NQF

S-001488 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series

FRET-CFP/YFP-C-NTE

S-000239 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator

FRET-CFP/YFP-C-OMF

S-001492 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81

FRET-CFP/YFP-C-ZHE

S-001494 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1

FRET-CFP/YFP-C-OFF

S-001490 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-FF for microscopes: BX53, BX63

FRET-CFP/YFP-C

S-000766 / 0

Out of Stock Backordered In Stock

FRET-CFP/YFP-C-NQF-ZERO

S-001489 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series

FRET-CFP/YFP-C-NTE-ZERO

S-000240 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator

1 of 2

TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
AmCyan1 458 489
CFP (ECFP) 433 475
Venus 516 528
YFP (yellow GFP) 513 530

Specifications

FRET-CFP/YFP-C-U03

S-000768 / 0

Out of Stock Backordered In Stock
This set is delivered unmounted and fits Olympus OFF cubes which is compatible with the BX3 (BX53, BX63) and IX3 Series (IX53, IX73, IX83) of Evident Scientific (Olympus) microscopes.

FRET-CFP/YFP-C-U03-ZERO

S-000769 / 0

Out of Stock Backordered In Stock
This set is delivered unmounted and fits Olympus OFF cubes which is compatible with the BX3 (BX53, BX63) and IX3 Series (IX53, IX73, IX83) of Evident Scientific (Olympus) microscopes.

FRET-CFP/YFP-C-NQF

S-001488 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series

FRET-CFP/YFP-C-NTE

S-000239 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator

FRET-CFP/YFP-C-OMF

S-001492 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81

FRET-CFP/YFP-C-ZHE

S-001494 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1

FRET-CFP/YFP-C-OFF

S-001490 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-FF for microscopes: BX53, BX63

FRET-CFP/YFP-C

S-000766 / 0

Out of Stock Backordered In Stock

FRET-CFP/YFP-C-NQF-ZERO

S-001489 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series

FRET-CFP/YFP-C-NTE-ZERO

S-000240 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator

1 of 2

TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.