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532 nm laser BrightLine® single-edge laser dichroic beamsplitter

Semrock Part Number: Di01-R532-25x36

IDEX Part Number: FL-117139  /


The perfect dichroic beamsplitters for the most popular lasers used in fluorescence imaging, including newer all-solid-state lasers. All beamsplitters in this category have exceptional reflectance at the laser wavelengths, and anti-reflection (AR) coatings to minimize imaging artifacts resulting from the coherent laser light. This laser dichroic beamsplitter is optimized for reflecting laser beams up to 2.5 mm in diameter while minimizing RWE.

Interested in an improved Flatness / RWE version? Consider Semrock's Super-resolution / TIRF dichroics:
1λ P-V RWE on 1mm Di03-R532-t1 , optimized for reflecting laser beams up to 10 mm in diameter while minimizing RWE
λ/5 P-V RWE on 3mm Di03-R532-t3, optimized for reflecting laser beams up to 22.5 mm in diameter while minimizing RWE
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Optical Specifications Value
Transmission Band 1 Tavg > 93% 545.3 – 1200 nm
Reflection Band 1 Rabs > 94% 514 – 532 nm
Reflection Band 1 (p-pol) Rabs > 90% 514 – 532 nm
Reflection Band 1 (s-pol) Rabs > 98% 514 – 532 nm
Edge Wavelength 1 542 nm
Laser Wavelengths 1 514.5 nm, 532 nm
General Filter Specifications Value
Angle of Incidence 45 degrees with a shift of 0.35%/degree (40 – 50 degrees)
Cone Half-angle 0.5 degrees
Optical Damage Rating Not tested
Steepness Steep
Physical Filter Specifications   Value
Transverse Dimensions (L x W) 25.2 mm x 35.6 mm
Transverse Tolerance ± 0.1 mm
Clear Aperture ≥ 80% (elliptical)
Scratch-Dig 60-40
Substrate Type Fused Silica
Filter Thickness (unmounted) 1.05 mm
Filter Thickness Tolerance (unmounted) ± 0.05 mm
Orientation Reflective surface marked with part number - Orient in direction of incoming light

Di03-R532-t3-25x36

FL-006803 / 0

Out of Stock Backordered In Stock
• Laser Dichroic Beamsplitter with edge at 538.4 nm
• Rabs > 94% 514 – 532 nm
• Ravg > 90% 350 – 514 nm
• Tavg > 93% 541.6 – 1200 nm
• Flatness / RWE: Super-resolution / TIRF
Compare Compare
More Info
Compare Compare

Di02-R532-25x36

FL-007047 / 0

Out of Stock Backordered In Stock
  • Laser Dichroic Beamsplitter with edge at 536.8 nm
  • Rabs > 94% 514 – 532 nm
  • Ravg > 90% 350 – 514 nm
  • Tavg > 93% 541.6 – 1200 nm
  • Flatness / RWE: Laser
Compare Compare
More Info
Compare Compare

Di03-R532-t1-25x36

FL-007081 / 0

Out of Stock Backordered In Stock
  • Laser Dichroic Beamsplitter with edge at 538.4 nm
  • Rabs > 94% 514 – 532 nm
  • Ravg > 90% 350 – 514 nm
  • Tavg > 93% 541.6 – 1200 nm
  • Flatness / RWE: Super-resolution / TIRF
    Compare Compare
    More Info
    Compare Compare
    Hide Show
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    1 of 1
    TopicDescription
    Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
    Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
    Flatness of Dichroic BeamsplittersDescribes the impact of non-flat dichroic beamsplitters on microscope image fidelity
    Laser Damage ThresholdProvides information for calculating laser damage threshold for pulsed and continuous wave lasers
    Optical Filters for Laser-based Fluorescence MicroscopesDiscusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection
    Journal Article
    TopicDescription
    Bringing Super-resolution to Fluorescence MicroscopyPrashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., BioPhotonics, May/June 2010
    Advances in fluorescence microscopy techniques allow users to break the defraction limit and visualize resolution similar to that of an electron microscope
    Fluorescence Imaging: Optical filters optimize laser-based fluorescence imaging systemsPrashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., Laser Focus World, January 2010
    Discusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection
    Perfecting TIRF OpticsPrashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., BioOptics World, Jan/Feb 2009
    Discusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection
    White Paper
    TopicDescription
    Optical Filters for Laser-based Fluorescence MicroscopesAnalysis of additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection with a focus on multiple-laser systems.
    Flatness of Dichroic Beamsplitters Affects Focus and Image QualityAnalysis of how the radius of curvature of a dichroic filter affects focal plane shift, spot size and image fidelity in fluorescence microscopy.
    Super-resolution MicroscopyDiscusses advancements in fluorescence microscopy techniques that allow users to break the defraction limit and visualize resolution similar to that of an electron microscope
    Application
    TopicDescription
    TIRF (Total Internal Reflection Fluorescence)An overview of TIRF microscopy, including solutions from Semrock and additional learning resources.
    Laser Scanning/Spinning Disk Confocal MicroscopyAn overview of confocal microscopy techniques and applications, including solutions from Semrock and additional learning resources.
    Laser-based InstrumentationAn review of common of laser-based instrumentation, including solutions from Semrock and additional learning resources.
    LinkDescription
    Request a DrawingLooking for an optical filter drawing file for this product? Request one now!
    Request Custom Optical FiltersContact us today to start a conversation about custom optical filters.
    Access Optical Filter WarrantyFind our warranty in our Terms & Conditions of Sale.
    Semrock Optical Filters RoHS CertificateRoHS, stands for Restriction of Hazardous Substances. The current RoHS Directive currently restricts the use of ten substances.
    Semrock Optical Filters REACH CertificateREACH is a regulation of the European Union, adopted to improve the protection of human health and the environment from the risks that can be posed by chemicals, while enhancing the competitiveness of the EU chemicals industry.
    Download Optical Filters 101 GuideFind an in-depth overview of optical filter principles, specifications, and applications. Request a digital copy of this guide now.
    Request Clean Filter Checklist PosterLooking to clean your optical filters? Need instructions? Request a printed copy of our Clean Filter Checklist Poster.

    Specifications

    Di03-R532-t3-25x36

    FL-006803 / 0

    Out of Stock Backordered In Stock
    • Laser Dichroic Beamsplitter with edge at 538.4 nm
    • Rabs > 94% 514 – 532 nm
    • Ravg > 90% 350 – 514 nm
    • Tavg > 93% 541.6 – 1200 nm
    • Flatness / RWE: Super-resolution / TIRF
    Compare Compare
    More Info
    Compare Compare

    Di02-R532-25x36

    FL-007047 / 0

    Out of Stock Backordered In Stock
    • Laser Dichroic Beamsplitter with edge at 536.8 nm
    • Rabs > 94% 514 – 532 nm
    • Ravg > 90% 350 – 514 nm
    • Tavg > 93% 541.6 – 1200 nm
    • Flatness / RWE: Laser
    Compare Compare
    More Info
    Compare Compare

    Di03-R532-t1-25x36

    FL-007081 / 0

    Out of Stock Backordered In Stock
    • Laser Dichroic Beamsplitter with edge at 538.4 nm
    • Rabs > 94% 514 – 532 nm
    • Ravg > 90% 350 – 514 nm
    • Tavg > 93% 541.6 – 1200 nm
    • Flatness / RWE: Super-resolution / TIRF
      Compare Compare
      More Info
      Compare Compare
      Hide Show
      {{compareProduct.AlternativeText}}
      {{compareProduct.AlternativeText}}

      / 

      1 of 1
      TopicDescription
      Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
      Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
      Flatness of Dichroic BeamsplittersDescribes the impact of non-flat dichroic beamsplitters on microscope image fidelity
      Laser Damage ThresholdProvides information for calculating laser damage threshold for pulsed and continuous wave lasers
      Optical Filters for Laser-based Fluorescence MicroscopesDiscusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection
      Journal Article
      TopicDescription
      Bringing Super-resolution to Fluorescence MicroscopyPrashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., BioPhotonics, May/June 2010
      Advances in fluorescence microscopy techniques allow users to break the defraction limit and visualize resolution similar to that of an electron microscope
      Fluorescence Imaging: Optical filters optimize laser-based fluorescence imaging systemsPrashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., Laser Focus World, January 2010
      Discusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection
      Perfecting TIRF OpticsPrashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., BioOptics World, Jan/Feb 2009
      Discusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection
      White Paper
      TopicDescription
      Optical Filters for Laser-based Fluorescence MicroscopesAnalysis of additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection with a focus on multiple-laser systems.
      Flatness of Dichroic Beamsplitters Affects Focus and Image QualityAnalysis of how the radius of curvature of a dichroic filter affects focal plane shift, spot size and image fidelity in fluorescence microscopy.
      Super-resolution MicroscopyDiscusses advancements in fluorescence microscopy techniques that allow users to break the defraction limit and visualize resolution similar to that of an electron microscope
      Application
      TopicDescription
      TIRF (Total Internal Reflection Fluorescence)An overview of TIRF microscopy, including solutions from Semrock and additional learning resources.
      Laser Scanning/Spinning Disk Confocal MicroscopyAn overview of confocal microscopy techniques and applications, including solutions from Semrock and additional learning resources.
      Laser-based InstrumentationAn review of common of laser-based instrumentation, including solutions from Semrock and additional learning resources.
      LinkDescription
      Request a DrawingLooking for an optical filter drawing file for this product? Request one now!
      Request Custom Optical FiltersContact us today to start a conversation about custom optical filters.
      Access Optical Filter WarrantyFind our warranty in our Terms & Conditions of Sale.
      Semrock Optical Filters RoHS CertificateRoHS, stands for Restriction of Hazardous Substances. The current RoHS Directive currently restricts the use of ten substances.
      Semrock Optical Filters REACH CertificateREACH is a regulation of the European Union, adopted to improve the protection of human health and the environment from the risks that can be posed by chemicals, while enhancing the competitiveness of the EU chemicals industry.
      Download Optical Filters 101 GuideFind an in-depth overview of optical filter principles, specifications, and applications. Request a digital copy of this guide now.
      Request Clean Filter Checklist PosterLooking to clean your optical filters? Need instructions? Request a printed copy of our Clean Filter Checklist Poster.

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