405/488/561/635/800-1050 nm lasers BrightLine® multi-edge multiphoton super-resolution / TIRF dichroic beamsplitter

Semrock Part Number: Di01-R405/488/561/635/800-t3-25x36

IDEX Part Number: FL-009918  /

Semrock
Innovative dichroic beamsplitter designed to simplify the combination of 1P confocal imaging and 2P excitation for multiphoton applications. This dichroic beamsplitter includes reflection bands for the most popular lasers used in fluorescence imaging, including all-solid-state lasers. The 405 nm reflection is optimal for imaging or uncaging applications. The 2P excitation region is ideal for femtosecond pulsed lasers such as Ti:Sapphire, OPO coupled, and Ytterbium-doped lasers, enabling deep tissue imaging with improved contrast. This multiphoton dichroic has low reflected GDD that helps minimize temporal pulse broadening of reflected laser beams, and have steep edges with very high and flat reflection and transmission bands.

Semrock's super-resolution / TIRF dichroics are available in two thicknesses and deliver industry-leading flatness for minimal focus shift and optical wavefront aberrations of the laser beam spot to enable popular imaging and super-resolution techniques such as TIRF, PALM, STORM, structured illumination, and STED.

2.5λ P-V RWE on 1 mm, optimized for reflecting laser beams up to 6 mm in diameter while minimizing RWE
λ/3 P-V RWE on 3 mm, optimized for reflecting laser beams up to 16.7 mm in diameter while minimizing RWE
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Optical Specifications Value
Transmission Band 1 Tavg > 92% 425 – 470 nm
Transmission Band 2 Tavg > 92% 508 – 540 nm
Transmission Band 3 Tavg > 92% 583 – 615 nm
Transmission Band 4 Tavg > 92% 671 – 725 nm
Reflection Band 1 Rabs > 94% 400 – 410 nm
Reflection Band 1 (p-pol) Rabs > 90% 400 – 410 nm
Reflection Band 1 (s-pol) Rabs > 98% 400 – 410 nm
Reflection Band 2 Rabs > 94% 483 – 493 nm
Reflection Band 2 (p-pol) Rabs > 90% 483 – 493 nm
Reflection Band 2 (s-pol) Rabs > 98% 483 – 493 nm
Reflection Band 3 Rabs > 94% 559 – 563 nm
Reflection Band 3 (p-pol) Rabs > 90% 559 – 563 nm
Reflection Band 3 (s-pol) Rabs > 98% 559 – 563 nm
Reflection Band 4 Rabs > 94% 635 – 647 nm
Reflection Band 4 (p-pol) Rabs > 90% 635 – 647 nm
Reflection Band 4 (s-pol) Rabs > 98% 635 – 647 nm
Reflection Band 5 Ravg > 94% 800 – 1050 nm
Reflection Band 5 (p-pol) Ravg > 90% 800 – 1050 nm
Reflection Band 5 (s-pol) Ravg > 98% 800 – 1050 nm
Edge Wavelength 1 420 nm
Edge Wavelength 2 501 nm
Edge Wavelength 3 577 nm
Edge Wavelength 4 662 nm
Edge Wavelength 5 735 nm
Laser Wavelengths 1 405 +/- 5 nm
Laser Wavelengths 2 488 +/- 5 nm
Laser Wavelength 3 559 +4/-0 nm, 561.4 nm
Laser Wavelengths 4 635 +7/-0 nm, 642 +/- 5 nm
Laser Wavelength 5 800 - 1050 nm
General Filter Specifications Value
Angle of Incidence 45 degrees with a shift of ~0.35%/degree (40 – 50 degrees)
Cone Half-angle 0.5 degrees
Optical Damage Rating 1 J/cm² @ 532 nm (10 ns pulse width)
Flatness / RWE Classification   Super-resolution / TIRF
Maximum Reflected Laser Beam Diameter 16.7 mm
Nominal Flatness < 0.19λ P-V per inch @ 632.8 nm
Reflected Wavefront Error < 0.33λ P-V RWE @ 632.8 nm
Steepness Standard
Group Delay Dispersion   ± 500 fs² over 800 - 1050 nm refl band for S-Pol and P-Pol
Physical Filter Specifications   Value
Transverse Dimensions (L x W) 25.2 mm x 35.6 mm
Transverse Tolerance ± 0.1 mm
Clear Aperture ≥ 80% (elliptical)
Scratch-Dig 60-40
Substrate Type Fused Silica
Filter Thickness (unmounted) 3.0 mm
Filter Thickness Tolerance (unmounted) ± 0.1 mm
Orientation Reflective surface marked with laser dot - Orient in direction of incoming light

Specifications