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BrightLine® full-multiband filter set, optimized for DAPI & FITC and other like fluorophores

Semrock Part Number: DA/FI-A-000

IDEX Part Number: S-000726  /

Semrock

This "full-multiband" filter set allows for simultaneous direct viewing or imaging of DAPI and FITC with a color camera.

  • Price Not Available.
  • Each
    US Domestic pricing – contact your local distributor for other currency list prices
  • Price Not Available.
  • 1,415.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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We now offer LFOV (large field of view) cube sets compatible with Nikon Eclipse Ti2 microscopes. Please contact us for more information.
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Set Components

FF01-387/480-25

FL-004187

Each
  • Tavg > 80% 381.5 – 392.5 nm & Tavg > 90% 466 – 495 nm
  • Center Wavelengths of 387 nm & 480 nm and GMBWs of 11 nm & 29 nm

FL-004187

FF01-433/530-25

FL-004229

Each
  • Tavg > 90% 414 – 452 nm & Tavg > 90% 510 – 550 nm
  • Center Wavelengths of 433 nm & 530 nm and GMBWs of 38 nm & 40 nm

FL-004229

FF403/502-Di01-25x36

FL-006945

Each
  • Dichroic Beamsplitter with edges at 403 nm & 502 nm
  • Ravg > 97.5% 370 – 393 nm & Ravg > 97.5% 466 – 495 nm
  • Tavg > 90% 414 – 452 nm & Tavg > 90% 510 – 550 nm

FL-006945

Optical Specifications Value
Blocking BrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.
General Filter Specifications Value
Optical Damage Rating Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.
Microscope Compatibility BrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Physical Filter Specifications   Value
Scratch-Dig 60-40
Dichroic Orientation Reflective coating side should face toward light source.

DA/FI-A-U03

S-411628 / 0

Out of Stock Backordered In Stock
This set is delivered unmounted and fits Olympus OFF cubes which is compatible with the BX3 (BX53, BX63) and IX3 Series (IX53, IX73, IX83) of Evident Scientific (Olympus) microscopes.
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DA/FI-A-NQF

S-000158 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series

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DA/FI-A-NTE

S-001334 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator

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DA/FI-A-OMF

S-001335 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81

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DA/FI-A-ZHE

S-001336 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1

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DA/FI-A-OFF

S-000159 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-FF for microscopes: BX53, BX63

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TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO™ option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
1,8-ANS 372 477
4-methylumbelliferone 357 441
5-carboxy-2,7-dichlorofluorescein 500 525
5-FAM (5-carboxyfluorescein) 492 518
6-carboxyrhodamine 6G 525 555
7-hydroxy-4-methylcoumarin 360 442
Acridine Orange (+DNA) 502 526
Acridine Yellow 470 550
Alexa Fluor® 405 401 422
Alexa Fluor® 430 431 541
Alexa Fluor® 488 499 520
Alexa Fluor® 500 503 525
AMCA (Aminomethylcoumarin) 350 448
AmCyan1 458 489
ATTO 488 500 525
ATTO 495 495 527
BCECF (pH 5.5) 481 518
BCECF (pH 9.0) 502 528
BD Horizon V450 406 449
BFP (EBFP) 380 440
BOBO™-1 461 484
BODIPY FL 505 512
BODIPY FL-MeOH 502 511
Calcein 494 514
Calcium Green-1 507 529
Calcofluor White 351 444
Cascade Blue™ 401 419
CellTrace calcein violet 400 452
CFP2 490 510
Cy2™ 492 507
CyQUANT GR-DNA 502 523
DAF-FM-NO 495 519
DAPI 359 461
Dendra2 (Green) 491 507
DiA 457 586
DiFMU-pH 9.0 358 450
DiO 475 500
Dronpa 502 516
DY-505-Phalloidin 503 530
DyLight 405 398 420
ecliptic pHluorin pH5.5 473 507
Emerald 491 511
FITC (Fluorescein) 495 519
FlAsH-CCPFCC 511 530
Fluo-3 506 527
Fluo-4 494 516
Fluorescein dextran 501 524
Fluorescein-pH 8.0 489 517
Fluoro-Emerald 494 518
FluoSpheres Yellow-Green fluorescent microspheres 503 514
FM 1-43 473 579
GFP (EGFP) 489 511
Green 496 496 520
Green 500 501 524
HCS LipidTOX Green neutral lipid stain 498 507
HCS LipidTOX Green phospholipidosis 504 536
HiLyte Fluor™ 405 403 428
HiLyte Fluor™ 488 497 526
LIVE-DEAD Fixable Green Dead Cell Stain 498 525
LIVE-DEAD Fixable Violet Dead Cell Stain 403 455
LysoSensor Blue 374 424
LysoSensor Green 448 502
LysoTracker Blue 373 422
LysoTracker Green 501 509
LysoTracker Yellow HCK-123 488 565
Magnesium Green 507 531
Marina Blue 363 461
mHoneyDew