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504/24 Avant™ Bandpass Filter

Semrock Part Number: AF01-504/24-25

IDEX Part Number: FL-429408  /

Semrock
For optimal filter set performance, pair this exciter with AF01-539/27 and AF521-Di01, which have been optimized for Yellow Fluorescent Protein (YFP), Venus, ATTO 488, ATTO 514, ATTO 520 and other key fluorophores. Precise edge placement of the steep spectral edges combined with deep complementary blocking maximize fluorophore performance compared to standard exciter and emitter pairs.

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  • 400.93 USD Each
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Optical Specifications Value
Transmission Band 1 Tavg > 93% 492.5 – 516.5 nm
Blocking Band 1   ODavg > 5 200 - 275 nm (Design specification - measurements are noise-floor limited)
Blocking Band 2 ODavg > 5 275 – 340 nm
Blocking Band 3 ODavg > 6 340 – 481 nm
Blocking Band 4 OD > 3.5 521 nm
Blocking Band 5   ODavg > 10 525.5 – 565 nm (Design specification - measurements are noise-floor limited)
Blocking Band 6 ODavg > 6 565 – 850 nm
Blocking Band 7 ODavg > 2 850 – 1100 nm
Center Wavelength 1 504 nm
Guaranteed Minimum Bandwidth 1 24 nm
FWHM Bandwidth 1 (nominal) 28 nm
General Filter Specifications Value
Angle of Incidence 0 ± 5 degrees
Cone Half-angle 7 degrees
Optical Damage Rating Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hrs.
Physical Filter Specifications   Value
Transverse Dimensions (diameter) 25 mm
Transverse Tolerance + 0.0 / – 0.1 mm
Filter Thickness (mounted) 5 mm
Filter Thickness Tolerance (mounted) ± 0.1 mm
Clear Aperture ≥ 21 mm
Scratch-Dig 60-40
Filter Thickness (unmounted) 2.0 mm
Filter Thickness Tolerance (unmounted) ± 0.1 mm
Orientation Arrow on ring indicates preferred direction of propagation of light
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
LinkDescription
Request a DrawingLooking for an optical filter drawing file for this product? Request one now!
Request Custom Optical FiltersContact us today to start a conversation about custom optical filters.
Access Optical Filter WarrantyFind our warranty in our Terms & Conditions of Sale.
Semrock Optical Filters RoHS CertificateRoHS, stands for Restriction of Hazardous Substances. The current RoHS Directive currently restricts the use of ten substances.
Semrock Optical Filters REACH CertificateREACH is a regulation of the European Union, adopted to improve the protection of human health and the environment from the risks that can be posed by chemicals, while enhancing the competitiveness of the EU chemicals industry.
Download Optical Filters 101 GuideFind an in-depth overview of optical filter principles, specifications, and applications. Request a digital copy of this guide now.
Request Clean Filter Checklist PosterLooking to clean your optical filters? Need instructions? Request a printed copy of our Clean Filter Checklist Poster.

Question 1: What is the AF01-504/24-25 used for?

Answer: The AF01-504/24-25 is a fluorescence bandpass filter designed to selectively transmit light around 504 nm while blocking unwanted wavelengths. It is commonly used in fluorescence microscopy, imaging systems, and analytical instrumentation.

Question 2: Is this filter intended to be used with other filters?

Answer: Yes. Semrock specifically recommends pairing this filter with, AF01-539/27 and AF521-Di01, when working with compatible fluorophores such as YFP, Venus, and ATTO dyes. Find the set here.

Question 3: How do I know if this is the right filter for my fluorophore?

Answer: This filter is optimized for applications involving short Stokes shift fluorophores such as:

  • YFP (Yellow Fluorescent Protein)
  • Venus
  • ATTO 514
  • ATTO 520

Users should compare the filter transmission range to their fluorophore excitation and emission spectra before selecting a filter.

Question 4: What does 504/24 mean?

Answer: The designation 504/24 indicates- Center wavelength: 504 nm and Bandwidth: 24 nm. This means the filter transmits a narrow spectral band centered near 504 nm while rejecting wavelengths outside that range.

Question 5: Why choose an Avant™ bandpass filter?

Answer: The Avant family combines:

  • Precise spectral edge placement
  • Steep transition edges
  • Deep blocking of unwanted wavelengths

This design helps maximize fluorophore performance and signal collection efficiency, particularly for short Stokes shift fluorophores.

Specifications

TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
LinkDescription
Request a DrawingLooking for an optical filter drawing file for this product? Request one now!
Request Custom Optical FiltersContact us today to start a conversation about custom optical filters.
Access Optical Filter WarrantyFind our warranty in our Terms & Conditions of Sale.
Semrock Optical Filters RoHS CertificateRoHS, stands for Restriction of Hazardous Substances. The current RoHS Directive currently restricts the use of ten substances.
Semrock Optical Filters REACH CertificateREACH is a regulation of the European Union, adopted to improve the protection of human health and the environment from the risks that can be posed by chemicals, while enhancing the competitiveness of the EU chemicals industry.
Download Optical Filters 101 GuideFind an in-depth overview of optical filter principles, specifications, and applications. Request a digital copy of this guide now.
Request Clean Filter Checklist PosterLooking to clean your optical filters? Need instructions? Request a printed copy of our Clean Filter Checklist Poster.

Question 1: What is the AF01-504/24-25 used for?

Answer: The AF01-504/24-25 is a fluorescence bandpass filter designed to selectively transmit light around 504 nm while blocking unwanted wavelengths. It is commonly used in fluorescence microscopy, imaging systems, and analytical instrumentation.

Question 2: Is this filter intended to be used with other filters?

Answer: Yes. Semrock specifically recommends pairing this filter with, AF01-539/27 and AF521-Di01, when working with compatible fluorophores such as YFP, Venus, and ATTO dyes. Find the set here.

Question 3: How do I know if this is the right filter for my fluorophore?

Answer: This filter is optimized for applications involving short Stokes shift fluorophores such as:

  • YFP (Yellow Fluorescent Protein)
  • Venus
  • ATTO 514
  • ATTO 520

Users should compare the filter transmission range to their fluorophore excitation and emission spectra before selecting a filter.

Question 4: What does 504/24 mean?

Answer: The designation 504/24 indicates- Center wavelength: 504 nm and Bandwidth: 24 nm. This means the filter transmits a narrow spectral band centered near 504 nm while rejecting wavelengths outside that range.

Question 5: Why choose an Avant™ bandpass filter?

Answer: The Avant family combines:

  • Precise spectral edge placement
  • Steep transition edges
  • Deep blocking of unwanted wavelengths

This design helps maximize fluorophore performance and signal collection efficiency, particularly for short Stokes shift fluorophores.

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