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    High-Speed Microscopy Tracks Millisecond Voltage Changes in Neurons of Awake Mice

    Article obtained from Photonics RSS Feed.

    University of California, Berkeley, researchers have built a microscope that can image the brain of an alert mouse 1000 times a second and record the passage of millisecond electrical pulses through neurons. The new imaging technique combines two-photon fluorescence microscopy and all-optical laser scanning in a microscope that can image a 2D slice through the neocortex of the mouse brain up to 3000 times per second. According to the researchers, that’s fast enough to trace electrical signals flowing through brain circuits.

    To track voltage changes in neurons, the researchers used a sensor that becomes fluorescent when the cell membrane depolarizes as a voltage signal propagates along the membrane. The scientists illuminated…

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    Mar, 23 2020 |

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