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Deconvolution is a computationally intensive image processing technique used to improve the contrast and sharpness of images captured with a light microscope. Light microscopes are diffraction limited, which means they are unable to resolve individual structures unless they are more than half the wavelength of light away from one another. Each point source below this diffraction limit is blurred by the microscope into what is known as a point spread function (PSF). With traditional wide-field fluorescence microscopy, out-of-focus light from areas above or below the focal plane causes additional blurring in the captured image. This becomes a problem when trying to identify or measure structures inside thicker samples.