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To enhance the imaging speed of two-photon microscopy without compromising resolution, researchers from The Chinese University of Hong Kong combined compressive imaging techniques with a multifocus laser illumination method that uses a digital micromirror device (DMD).
The DMD generates 5 to 30 points of focused laser light on randomly selected locations within a specimen. The position and intensity of each point of light are controlled by a binary hologram that is projected onto the device. During each measurement, the DMD reflashes the hologram to change the position of each focus and records the intensity of the two-photon fluorescence with a single-pixel detector.
The speed of the DMD was limited by the rate at which the…READ MORE