| Dead Volume One of the concerns you will have as a chromatographer is about the dead volume existing in your system. Dead volume is described as a small space within your HPLC system where remixing of the sample may occur or where your sample can be diluted with mobile phase. Dead volume can cause critical problems in your analysis of chemical compounds, as it may bring about band broadening or split peaks in the end result, making it difficult to obtain good data on your samples. One of the largest sources of dead volume in your system will be at the unions, where two pieces of tubing are coming together or a piece of tubing is attached to a piece of equipment. You must keep this in mind when making connections throughout your system. |
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